• DRAMP ID

    • DRAMP01055
    • Peptide Name

    • P. americana AMP (Pa-AMP-1; PAFP-S; Cys-rich; Plant defensin)
    • Source

    • Phytolacca americana (American pokeweed) (Phytolacca decandra)
    • Family

    • Belongs to the AMP family
    • Gene

    • Not found
    • Sequence

    • AGCIKNGGRCNASAGPPYCCSSYCFQIAGQSYGVCKNR
    • Sequence Length

    • 38
    • Protein Existence

    • Protein level
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal
    • Target Organism

      • Gram-positive bacteria: Bacillus megaterium (IC50=8 µg/ml), Staphyanococcus sp. (IC50=11 µg/ml).
      • Fungi: Alternaria panax, Fusarium sp., Rhizoctonia solani.
    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Not found
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Beta strand (3 strands; 13 residues)
    • Structure Description

    • The global fold involves a cystine-knotted three-stranded antiparallel beta-sheet (residues 8-10, 23-27, 32-36), a flexible loop (residues 14-19), and four beta-reverse turns (residues 4-8, 11-14, 19-22, 28-32). This structure features all the characteristics of the knottin fold. It is the first structural model of an antifungal peptide that adopts a knottin-type structure.
    • Helical Wheel Diagram

    • DRAMP01055 helical wheel diagram
    • PDB ID

    • 1DKC resolved by NMR.
  • 1DKC-> 
    • Predicted Structure

    • There is no predicted structure for DRAMP01055.
    • Formula

    • C163H253N51O51S6
    • Absent Amino Acids

    • DEHLMTW
    • Common Amino Acids

    • CG
    • Mass

    • 3935.47
    • PI

    • 8.9
    • Basic Residues

    • 4
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 8
    • Net Charge

    • +4
    • Boman Index

    • -48.54
    • Hydrophobicity

    • -0.232
    • Aliphatic Index

    • 38.68
    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 4845
    • Absorbance 280nm

    • 130.95
    • Polar Residues

    • 22

DRAMP01055

DRAMP01055 chydropathy plot
    • Function

    • Possesses antifungal activity.
    • Tissue specificity

    • Seed specific.
    • Domain

    • The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.
    • PTM

    • Contains three disulfide bonds
  • ·Literature 1
    • Title

    • Purification, characterization, and molecular cloning of the gene of a seed-specific antimicrobial protein from pokeweed.
    • Reference

    • Plant Physiol. 2000 Apr;122(4):1015-1024.
    • Author

    • Liu Y, Luo J, Xu C, Ren F, Peng C, Wu G, Zhao J.
  • ·Literature 2
    • Title

    • Solution structure of PAFP-S: a new knottin-type antifungal peptide from the seeds of Phytolacca americana.
    • Reference

    • Biochemistry. 2001 Sep 18;40(37):10973-10978.
    • Author

    • Gao GH, Liu W, Dai JX, Wang JF, Hu Z, Zhang Y, Wang DC.