• • DRAMP ID

  • DRAMP01098

• • Peptide Name

  • Bombinin-like peptide 3 (BLP-3; toads, amphibians, animals)

• • Source

  • Bombina orientalis (Oriental fire-bellied toad)

• • Family

  • Belongs to the bombinin family

• • Gene

  • Not found

• • Sequence

  • GIGAAILSAGKSALKGLAKGLAEHF

• • Sequence Length

  • 25

• • UniProt Entry

  • P29004

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram-

• • Target Organism

  • • [Ref.1744108]Gram-postive bacterium:Bacillus megaterium BmII (MIC=0.3 µM), Staphylococcus aureus Cowan1 (MIC=1.7 µM);
    • Gram-negative bacterium:59% inhibition Neisseria meningitidis 118V C (MIC=20 µg/ml), 99% inhibition Neisseria meningitidis 15240 IAI (MIC=20 µg/ml), 99% inhibition Neisseria meningitidis 126E IC1 (MIC=20 µg/ml), 99% inhibition Neisseria gonorrhoeoe F62 (MIC=20 µg/ml), 97% inhibitionNeisseria Iactamica 15323 (MIC=20 µg/ml), 100% inhibition Neisseria Iactamica 15215A (MIC=20 µg/ml), 97% inhibition Neisseria cinerea 15461 (MIC=20 µg/ml), Escherichia coli D21 (MIC=1.7 µM), Escherichia coli D22 (MIC=1.5 µM), Yersinia pseudotubercolosis (MIC=0.6 µM), Pseudomonas aeruginosa ATCC 15692 (MIC=9.2 µM);
    • Fungus:Candida albicans (MIC=0.4 µM).

• • Hemolytic Activity

  • • [Ref:1744108] No significant hemolytic activity was found which suggests a selectivity for prokaryotic over eukaryotic membranes.

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Alpha helix

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• Please click DRAMP01098_predicted_structure.pdb to download.

DRAMP01098

• • Function

  • Has antimicrobial activity, but no hemolytic activity. Preference on killing Gram-negative non-enteric bacteria.

• • Tissue specificity

  • Expressed by the skin glands.

• ·Literature 1

• • Title

  • Bombinin-like peptides with antimicrobial activity from skin secretions of the Asian toad, Bombina orientalis.

• • Pubmed ID

  • 1744108

• • Reference

  • J Biol Chem. 1991 Dec 5;266(34):23103-23111.

• • Author

  • Gibson BW, Tang DZ, Mandrell R, Kelly M, Spindel ER.