General Information

    • DRAMP ID

    • DRAMP01152

    • Peptide Name

    • Uperin-3.6 (toads, amphibians, animals)

    • Source

    • Uperoleia mjobergii (Australian toadlet)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GVIDAAKKVVNVLKNLP

    • Sequence Length

    • 17

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Bacillus cereus (MIC=25 µg/ml), Lactococcus lactis (MIC=3 µg/ml), Listeria innocua (MIC=50 µg/ml), Micrococcus luteus (MIC=25 µg/ml), Staphylococcus aureus (MIC=25 µg/ml), Staphylococcus epidermidis (MIC=12.5 µg/ml), Streptococcus uberis (MIC=12.5 µg/ml).(Ref.2)

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • In 50% (by vol.) trifluoroethanol, an NMR study and structure calculations indicate that uperin 3.6 adopts a well-defined amphipathic alpha-helix with distinct hydrophilic and hydrophobic faces.(Ref.1)

    • Helical Wheel Diagram

    • DRAMP01152 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01152.

Physicochemical Information

    • Formula

    • C81H144N22O22

    • Absent Amino Acids

    • CEFHMQRSTWY

    • Common Amino Acids

    • V

    • Mass

    • 1778.17

    • PI

    • 9.7

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • -3.17

    • Hydrophobicity

    • 0.488

    • Aliphatic Index

    • 148.82

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP01152

Comments Information

    • Function

    • Shows antibacterial activity gaginst several Gram-positive bacteria. This peptide could inhibit HIV infection at a concentration that is also toxic to T cells (J Virol 2005; 79

Literature Information

  • ·Literature 1

    • Title

    • The solution structure of uperin 3.6, an antibiotic peptide from the granular dorsal glands of the Australian toadlet, Uperoleia mjobergii.

    • Reference

    • J Pept Res. 1999 Aug;54(2):137-145.

    • Author

    • Chia BC, Carver JA, Mulhern TD, Bowie JH.
  • ·Literature 2

    • Title

    • Host-defence peptides of Australian anurans: structure, mechanism of action and evolutionary significance.

    • Reference

    • Peptides. 2004 Jun;25(6):1035-1054.

    • Author

    • Apponyi MA, Pukala TL, Brinkworth CS, Maselli VM, Bowie JH, Tyler MJ, Booker GW, Wallace JC, Carver JA, Separovic F, Doyle J, Llewellyn LE.