General Information

    • DRAMP ID

    • DRAMP01209

    • Peptide Name

    • Pleurain-A2 (Pleurain A2; Frogs, amphibians, animals)

    • Source

    • Rana pleuraden (Yunnan pond frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • SIITMTKEAKLPQSWKQIACRLYNTC

    • Sequence Length

    • 26

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.17764786]Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC=60 µg/ml), Helicobacter pylori NCTC 11637 (MIC=30 µg/ml);
      • Gram-positive bacteria: Bacillus dysenteriae (MIC=60 µg/ml), Staphylococcus aureus ATCC 2592 (MIC=15 µg/ml);
      • Yeast: Candida albicans ATCC 2002 (MIC=30 µg/ml).

    • Hemolytic Activity

      • [Ref:17764786] It has little hemolytic activity on red blood cells even with peptide concentration up to 200 μg/ml

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys20 and Cys26)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys20 and Cys26.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01209 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C133H220N36O38S3

    • Absent Amino Acids

    • DFGHV

    • Common Amino Acids

    • IKT

    • Mass

    • 3027.61

    • PI

    • 9.3

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +3

    • Boman Index

    • -35.29

    • Hydrophobicity

    • -0.235

    • Aliphatic Index

    • 82.69

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 7115

    • Absorbance 280nm

    • 284.6

    • Polar Residues

    • 9

DRAMP01209

DRAMP01209 chydropathy plot

Comments Information

    • Function

    • Has little hemolytic activity on red blood cells.

    • Tissue specificity

    • Expressed by the skin dorsal glands.

Literature Information

  • ·Literature 1

    • Title

    • A new family of antimicrobial peptides from skin secretions of Rana pleuraden.

    • Reference

    • Peptides. 2007 Oct;28(10):2069-2074.

    • Author

    • Wang X, Song Y, Li J, Liu H, Xu X, Lai R, Zhang K.