General Information

    • DRAMP ID

    • DRAMP01222

    • Peptide Name

    • Palustrin-2AJ1 (PL2AJ1; Frogs, amphibians, animals)

    • Source

    • Amolops jingdongensis (Chinese torrent frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GFMDTAKNVAKNVAVTLIDKLRCKVTGGC

    • Sequence Length

    • 29

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.21816202]Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC=20 µM), Enterococcus faecalis ATCC29212 (MIC=20 µM);
      • Gram-negative bacteria: Escherichia coli ATCC25922 (MIC=13 µM), Bacillus pyocyaneus CMCCB1010 (MIC=20 µM), Pseudomonas aeruginosa ATCC27853 (MIC=33 µM), Bacillus dysenteriae (MIC=50 µM).

    • Hemolytic Activity

      • [Ref:21816202] IC50=135 μM against human and rabbit erythrocytes

    • Cytotoxicity

      • [Ref.21816202] Cytotoxicity: the K562 (IC50=58μM) and HT29 mammalian cell lines(IC50=57μM).

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys23 and Cys29)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys23 and Cys29.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01222 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C131H226N38O39S3

    • Absent Amino Acids

    • EHPQSWY

    • Common Amino Acids

    • KV

    • Mass

    • 3053.65

    • PI

    • 9.31

    • Basic Residues

    • 5

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +3

    • Boman Index

    • -28.49

    • Hydrophobicity

    • 0.228

    • Aliphatic Index

    • 90.69

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.46

    • Polar Residues

    • 10

DRAMP01222

DRAMP01222 chydropathy plot

Comments Information

    • Function

    • Displays broad-spectrum antibacterial activity against a range of Gram-positive and Gram-negative bacteria. Has hemolytic activity, low cytotoxicity and low antioxidant activity.

    • Tissue specificity

    • Expressed by the skin glands.

    • PTM

    • One disulfide bond (C23-C29) and Cleavage on pair of basic residues.

Literature Information

  • ·Literature 1

    • Title

    • Two novel families of antimicrobial peptides from skin secretions of the Chinese torrent frog, Amolops jingdongensis.

    • Reference

    • Biochimie. 2012 Feb;94(2):328-334.

    • Author

    • Chen Z, Yang X, Liu Z, Zeng L, Lee W, Zhang Y.