General Information

    • DRAMP ID

    • DRAMP01257

    • Peptide Name

    • Dermadistinctin-K (DD K; Frogs, amphibians, animals)

    • Source

    • Phyllomedusa distincta (Monkey frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLWSKIKAAGKEAAKAAAKAAGKAALNAVSEAV

    • Sequence Length

    • 33

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiprotozoal

    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus (MIC=4.7 µM), Enterococcus faecalis (MIC>19 µM);Gram-negative bacteria: Pseudomonas aeruginosa (MIC=2.4 µM), Escherichia coli (MIC=0.6 µM).
      • Fungi: Candida tropicalis (MIC=10.1 µM), Candida guilliermondii (MIC=20.3 µM), Candida albicans (MIC=20.3 µM), Candida albicans ATCC 1023 (MIC=10.1 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • liposomes

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01257 helical wheel diagram
  • 2K9B-> 

    • Predicted Structure

Physicochemical Information

    • Formula

    • C140H239N41O41

    • Absent Amino Acids

    • CDFHMPQRTY

    • Common Amino Acids

    • A

    • Mass

    • 3152.69

    • PI

    • 10

    • Basic Residues

    • 6

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 19

    • Net Charge

    • +4

    • Boman Index

    • -8.84

    • Hydrophobicity

    • 0.191

    • Aliphatic Index

    • 92.42

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 171.88

    • Polar Residues

    • 6

DRAMP01257

DRAMP01257 chydropathy plot

Comments Information

    • Function

    • Also has antiprotozoal activity against Trypanosoma cruzi. Decreases viability of murine peritoneal cells. Fuses to, and disrupts liposomes.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides from the Brazilian frog Phyllomedusa distincta.

    • Reference

    • Peptides. 1999;20(6):679-686.

    • Author

    • Batista CV, da Silva LR, Sebben A, Scaloni A, Ferrara L, Paiva GR, Olamendi-Portugal T, Possani LD, Bloch C Jr.