General Information

    • DRAMP ID

    • DRAMP01341

    • Peptide Name

    • Amolopin-1b (Frogs, amphibians, animals)

    • Source

    • Amolops loloensis (Rufous-spotted torrent frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • FLPLAVSLAANFLPKLFCKITKKC

    • Sequence Length

    • 24

    • Protein Existence

    • Homology

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus (MIC=5.50 µg/ml), Bacillus dysenteriae (MIC=2.20 µg/ml);
      • Gram-negative bacterium: Escherichia coli (MIC=6.50 µg/ml).
      • Yeast: Candida albicans (MIC=7.50 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • [Ref.17000029]Display cytotoxicity against HepG2(IC50=58 mg/ml).

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization(Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bridge between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01341 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C128H209N29O28S2

    • Absent Amino Acids

    • DEGHMQRWY

    • Common Amino Acids

    • L

    • Mass

    • 2666.36

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +4

    • Boman Index

    • 15.68

    • Hydrophobicity

    • 0.946

    • Aliphatic Index

    • 122.08

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 5

DRAMP01341

DRAMP01341 chydropathy plot

Comments Information

    • Function

    • Has antibacterial against Gram-positive and Gram-negative bacteria. Also has antifungal activity against C. albicans.

Literature Information

  • ·Literature 1

    • Title

    • Two families of antimicrobial peptides with multiple functions from skin of rufous-spotted torrent frog, Amolops loloensis.

    • Reference

    • Peptides. 2006 Dec;27(12):3085-3091.

    • Author

    • Lu Y, Li J, Yu H, Xu X, Liang J, Tian Y, Ma D, Lin G, Huang G, Lai R.