General Information

    • DRAMP ID

    • DRAMP01347

    • Peptide Name

    • Prepromelittin-related peptide (Frogs, amphibians, animals)

    • Source

    • Rana tagoi (Tago frog)

    • Family

    • Not found

    • Gene

    • prMRP

    • Sequence

    • AIGSILGALAKGLPTLISWIKNR

    • Sequence Length

    • 23

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.12804591]Gram-positive bacterium: Staphylococcus aureus (MIC=3.5 µM);
      • Gram-negative bacterium: Escherichia coli (MIC=20 µM).
      • Yeast: Candida albicans (MIC=19 µM).
      • EL4 T-lymphoma cells (MIC=14 µM).
      • [Ref.27271216]Gram-positive bacteria : Staphylococcuss aureus (MIC=6.25 μM), Staphylococcus epidermidis (MIC=3.13 μM), Bacillus subtilis (MIC=3.13 μM);
      • Gram-negative bacteria : Escherichia coli (MIC=25 μM), Pseudomonas aeruginosa (MIC=12.5 μM), Klebsiella pneumoniae (MIC=12.5 μM)

    • Hemolytic Activity

      • [Ref.27271216] 100% hemolysis at 20 μM against human red blood cells

    • Cytotoxicity

      • [Ref.12804591]Display cytotoxic against L929 fibroblasts(LC50=20 μM);EL4 T-lymphoma cells(LC50=14 μM).
      • [Ref.27271216]L929 cells:Cell viability is approximately 10% at the concentration of 6.25 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01347 helical wheel diagram

    • PDB ID

    • 6dst resolved by NMR (related PDB_ID)

    • Predicted Structure

Physicochemical Information

    • Formula

    • C111H190N30O28

    • Absent Amino Acids

    • CDEFHMQVY

    • Common Amino Acids

    • IL

    • Mass

    • 2392.91

    • PI

    • 11.17

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 12

    • Net Charge

    • +3

    • Boman Index

    • 7.91

    • Hydrophobicity

    • 0.73

    • Aliphatic Index

    • 148.7

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 250

    • Polar Residues

    • 7

DRAMP01347

DRAMP01347 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against C. albicans.

Literature Information

  • ·Literature 1

    • Title

    • A melittin-related peptide from the skin of the Japanese frog, Rana tagoi, with antimicrobial and cytolytic properties

    • Reference

    • Biochem Biophys Res Commun. 2003 Jun 27;306(2):496-500.

    • Author

    • Conlon JM, Sonnevend A, Patel M, Camasamudram V, Nowotny N, Zilahi E, Iwamuro S, Nielsen PF, Pál T
  • ·Literature 2

    • Title

    • Expression of genes encoding antimicrobial and bradykinin-related peptides in skin of the stream brown frog Rana sakuraii

    • Reference

    • Peptides. 2007;28(3):505-514.

    • Author

    • Suzuki H, Iwamuro S, Ohnuma A, Coquet L, Leprince J, Jouenne T, Vaudry H, Taylor CK, Abel PW, Conlon JM.
  • ·Literature 3

    • Title

    • Design of an α-helical antimicrobial peptide with improved cell selective and potent anti-biofilm activity

    • Reference

    • Sci Rep. 2016 Jun 8;6:27394.

    • Author

    • Shi-KunZhang, Jin-wen Song, FengGong, Su-Bo Li, Hong-Yu Chang, Hui-MinXie, Hong-WeiGao, Ying-XiaTan & Shou-Ping Ji