General Information

    • DRAMP ID

    • DRAMP01353

    • Peptide Name

    • Tigerinin-4 (Frogs, amphibians, animals)

    • Source

    • Rana tigerina (Indian bull frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RVCYAIPLPIC

    • Sequence Length

    • 11

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacterium: Staphylococcus aureus ATCC 8530 (MIC=50 µg/ml);
      • Gram-negative bacteria: Escherichia coli (MIC=60 µg/ml), Pseudomonas putida NCIM 2102 (MIC=60 µg/ml).
      • Fungi: Saccharomyces cerevisiae ATCC 8530 (MIC=100 µg/ml).

    • Hemolytic Activity

      • [Ref.11031261] It exhibits no hemolytic activity up to a concentration of 200 μg/ml against rat erythrocytes.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation;Cyclization (Cys3 and Cys11)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys3 and Cys11.

    • Stereochemistry

    • L

    • Structure

    • Beta strand-turn

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01353 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C57H94N14O13S2

    • Absent Amino Acids

    • DEFGHKMNQSTW

    • Common Amino Acids

    • CIP

    • Mass

    • 1247.58

    • PI

    • 8.06

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +1

    • Boman Index

    • 8.11

    • Hydrophobicity

    • 1.346

    • Aliphatic Index

    • 141.82

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 1615

    • Absorbance 280nm

    • 161.5

    • Polar Residues

    • 3

DRAMP01353

Comments Information

    • Function

    • Has antibacterial against Gram-positive and Gram-negative bacteria. Also has antifungal activity against C. albicans.

Literature Information

  • ·Literature 1

    • Title

    • Tigerinins: novel antimicrobial peptides from the Indian frog Rana tigerina.

    • Reference

    • J Biol Chem. 2001 Jan 26;276(4):2701-2707.

    • Author

    • Sai KP, Jagannadham MV, Vairamani M, Raju NP, Devi AS, Nagaraj R, Sitaram N.