General Information

    • DRAMP ID

    • DRAMP01354

    • Peptide Name

    • Peptide leucine arginine (pLR; Frogs, amphibians, animals)

    • Source

    • Rana pipiens (Northern leopard frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • LVRGCWTKSYPPKPCFVR

    • Sequence Length

    • 18

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal

    • Target Organism

      • [Ref.11099505]Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=20 µM), Staphylococcus lentus (MIC=50 µM), Micrococcus luteus (MIC=10 µM);
      • Fungi: Candida tropicalis (MIC=11 µM), Candida guillermondii (MIC=6.6 µM), Phytophthora nicotianae spores (MIC=75 µM).

    • Hemolytic Activity

      • [Ref:11099505] It has 2.5% hemolytic activity at 100 μM against human red blood

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys5 and Cys15.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01354 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01354.

Physicochemical Information

    • Formula

    • C99H153N27O22S2

    • Absent Amino Acids

    • ADEHIMNQ

    • Common Amino Acids

    • P

    • Mass

    • 2137.59

    • PI

    • 9.85

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +4

    • Boman Index

    • -25.24

    • Hydrophobicity

    • -0.317

    • Aliphatic Index

    • 53.89

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 7115

    • Absorbance 280nm

    • 418.53

    • Polar Residues

    • 6

DRAMP01354

DRAMP01354 chydropathy plot

Comments Information

    • Function

    • Mast cell degranulating peptide. Antiproliferative activity against human breast and ovarian tumor cell lines in vitro. Inhibits granulopoiesis in rat in vitro. Causes histamine release from rat peritoneal mast cells in vitro. Has antibacterial activity against Gram-positive bacteria

Literature Information

  • ·Literature 1

    • Title

    • Peptide leucine arginine, a potent immunomodulatory peptide, isolated and structurally characterized from the skin of the Northern Leopard frog, Rana pipiens.

    • Reference

    • J. Biol. Chem. 2001;276:10145-10152.

    • Author

    • Salmon A.L, Cross L.J.M, Irvine A.E, Lappin T.R.J, Dathe M, Krause G, Canning P, Thim L, Beyermann M, Rothemund S, Bienert M, Shaw C.