General Information

    • DRAMP ID

    • DRAMP01355

    • Peptide Name

    • Ranalexin (Frogs, amphibians, animals)

    • Source

    • Lithobates catesbeiana (American bullfrog) (Rana catesbeiana)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • FLGGLIKIVPAMICAVTKKC

    • Sequence Length

    • 20

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacterium: Staphylococcus aureus (MIC=4 µg/ml);
      • Gram-negative bacteria: Escherichia coli (MIC=32 µg/ml), Pseudomonas aeruginosa (MIC=128 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys14 and Cys20)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys14 and Cys20.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01355 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C97H169N23O22S3

    • Absent Amino Acids

    • DEHNQRSWY

    • Common Amino Acids

    • IK

    • Mass

    • 2105.73

    • PI

    • 9.39

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +3

    • Boman Index

    • 26.85

    • Hydrophobicity

    • 1.4

    • Aliphatic Index

    • 136.5

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 6.58

    • Polar Residues

    • 5

DRAMP01355

DRAMP01355 chydropathy plot

Comments Information

    • Function

    • Potent microbicidal activity, active against S.aureus and E.coli. It acts as well as a membrane-disruptive agent at higher concentrations.

    • Tissue specificity

    • Expressed by the skin dorsal glands.

    • Developmental stage

    • Expression starts at metamorphosis and continues into adulthood.

    • PTM

    • Contains one disulfide bond 14-20.

Literature Information

  • ·Literature 1

    • Title

    • Ranalexin. A novel antimicrobial peptide from bullfrog (Rana catesbeiana) skin, structurally related to the bacterial antibiotic, polymyxin.

    • Reference

    • J Biol Chem. 1994 Apr 8;269(14):10849-10855.

    • Author

    • Clark DP, Durell S, Maloy WL, Zasloff M.
  • ·Literature 2

    • Title

    • Solution structure of the antimicrobial peptide ranalexin and a study of its interaction with perdeuterated dodecylphosphocholine micelles.

    • Reference

    • Eur J Biochem. 1998 Apr 1;253(1):221-228.

    • Author

    • Vignal E, Chavanieu A, Roch P, Chiche L, Grassy G, Calas B, Aumelas A.