General Information

    • DRAMP ID

    • DRAMP01386

    • Peptide Name

    • Odorranain-P1a (OdP1a; Brevinin-1HS1; Brevinin-1-OA2; Frogs, amphibians, animals)

    • Source

    • Odorrana grahami (Yunnanfu frog) (Rana grahami)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Brevinin subfamily.

    • Gene

    • Not found

    • Sequence

    • VIPFVASVAAEMMQHVYCAASKKC

    • Sequence Length

    • 24

    • Protein Existence

    • Transcript level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.17272268] Gram-negative bacterium: Escherichia coli (MIC=3.50 μg/ml);
      • Gram-positive bacteria: Staphylococcus aureus (MIC=3.50 μg/ml), Bacillus subtilis (MIC=1.75 μg/ml).
      • Yeast: Candida albicans (MIC=1.75 μg/ml).

    • Hemolytic Activity

      • [Ref.17272268] RCH=11.2 ± 2.9% against rabbit red blood cells. RCH, red cell hemolysis.

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01386 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C114H183N29O31S4

    • Absent Amino Acids

    • DGLNRTW

    • Common Amino Acids

    • A

    • Mass

    • 2584.12

    • PI

    • 8.02

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +2

    • Boman Index

    • 5.32

    • Hydrophobicity

    • 0.808

    • Aliphatic Index

    • 85.42

    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 1615

    • Absorbance 280nm

    • 70.22

    • Polar Residues

    • 5

DRAMP01386

DRAMP01386 chydropathy plot

Comments Information

    • Function

    • Odorranain-P1a exhibites antimicrobial activities against all of the tested microbes including Gram-positive and Gram-negative bacteria and fungi. Also has hemolytic activity against rabbit red cell.

Literature Information

  • ·Literature 1

    • Title

    • Anti-infection peptidomics of amphibian skin.

    • Reference

    • Mol Cell Proteomics. 2007 May;6(5):882-894.

    • Author

    • Li J, Xu X, Xu C, Zhou W, Zhang K, Yu H, Zhang Y, Zheng Y, Rees HH, Lai R, Yang D, Wu J.