General Information

    • DRAMP ID

    • DRAMP01414

    • Peptide Name

    • Nigrocin-2ISa (Frogs, amphibians, animals)

    • Source

    • Odorrana ishikawae (Ishikawa's frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GIFSTVFKAGKGIVCGLTGLC

    • Sequence Length

    • 21

    • Protein Existence

    • Homology

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacterium: Escherichia coli (MIC=25 µM);
      • Gram-positive bacteria: Staphylococcus aureus (MIC=3.1 µM), Staphylococcus aureus (MRSA) (MIC=12.5 µM), Bacillus subtilis (MIC=12.5 µM).
      • Yeast: Candida albicans (MIC=50 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys15 and Cys21)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys15 and Cys21.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01414 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C94H155N23O25S2

    • Absent Amino Acids

    • DEHMNPQRWY

    • Common Amino Acids

    • G

    • Mass

    • 2071.52

    • PI

    • 8.9

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • 23.15

    • Hydrophobicity

    • 1.21

    • Aliphatic Index

    • 106.67

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 6.25

    • Polar Residues

    • 10

DRAMP01414

DRAMP01414 chydropathy plot

Comments Information

    • Function

    • Has antibacterial and antifungal activity.

Literature Information

  • ·Literature 1

    • Title

    • Identification and characterization of antimicrobial peptides from the skin of the endangered frog Odorrana ishikawae.

    • Reference

    • Peptides. 2011 Apr;32(4):670-676.

    • Author

    • Iwakoshi-Ukena E, Ukena K, Okimoto A, Soga M, Okada G, Sano N, Fujii T, Sugawara Y, Sumida M.