General Information

    • DRAMP ID

    • DRAMP01469

    • Peptide Name

    • Esculentin-2-Ala (Frogs, amphibians, animals)

    • Source

    • Amolops loloensis

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GIFALIKTAAKFVGKNLLKQAGKAGLEHLACKANNQC

    • Sequence Length

    • 37

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.19843479]Gram-positive bacteria: Staphylococcus aureus ATCC2592 (MIC=2.5 µg/mL), Bacillus dysenteriae (MIC=7.5 µg/mL), Bacillus cereus (MIC=7.5 µg/mL);
      • Gram-negative bacteria: Escherichia coli ATCC25922 (MIC=12.5 µg/mL), Bacillus pumilus (MIC=2.5 µg/mL), Acinetobacter calcoaceticus (MIC=25 µg/mL), Psecdomonas aeruginosa (MIC=50 µg/mL);
      • Yeast: Candida albicans ATCC2002 (MIC=2.5 µg/mL)

    • Hemolytic Activity

      • [Ref:19843479] It has 22% hemolytic activity at 100 μg/mL against rabbit red blood cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys31 and Cys37)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys31 and Cys37.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01469 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01469.

Physicochemical Information

    • Formula

    • C173H290N50O46S2

    • Absent Amino Acids

    • DMPRSWY

    • Common Amino Acids

    • A

    • Mass

    • 3870.63

    • PI

    • 9.79

    • Basic Residues

    • 7

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 17

    • Net Charge

    • +6

    • Boman Index

    • -14.91

    • Hydrophobicity

    • 0.149

    • Aliphatic Index

    • 100.54

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.47

    • Polar Residues

    • 10

DRAMP01469

DRAMP01469 chydropathy plot

Comments Information

    • Function

    • Esculentin-2-Ala exhibits hemolytic activity against rabbit red blood cells.

Literature Information

  • ·Literature 1

    • Title

    • Five novel antimicrobial peptides from skin secretions of the frog, Amolops loloen.

    • Reference

    • Comp Biochem Physiol B Biochem Mol Biol. 2010 Jan;155(1):72-76.

    • Author

    • Wang M, Wang Y, Wang A, Song Y, Ma D, Yang H, Ma Y, Lai R.