General Information

    • DRAMP ID

    • DRAMP01517

    • Peptide Name

    • Esculentin-2P (Frogs, amphibians, animals)

    • Source

    • Rana pipiens (northern leopard frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GFSSIFRGVAKFASKGLGKDLARLGVNLVACKISKQC

    • Sequence Length

    • 37

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-, Antiviral

    • Target Organism

      • [Ref.10651828]Gram-negative bacterium: Escherichia coli (MIC=10 μM).
      • [Ref.11601906]Virus:
      • Frog Virus 3: 90% inhibition at 500 µM;
      • Channel Catfish Virus: 90% inhibition at 50 µM.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization of a C-terminal Cys residue (forming a disulfide bond)

    • Nonterminal Modifications and Unusual Amino Acids

    • There is a disulfide bond between Cys31 and Cys37.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01517 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C173H290N50O46S2

    • Absent Amino Acids

    • EHMPTWY

    • Common Amino Acids

    • GK

    • Mass

    • 3870.63

    • PI

    • 10.21

    • Basic Residues

    • 7

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 16

    • Net Charge

    • +6

    • Boman Index

    • -27.01

    • Hydrophobicity

    • 0.357

    • Aliphatic Index

    • 97.57

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.47

    • Polar Residues

    • 12

DRAMP01517

DRAMP01517 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-negative bacteria.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Inactivation of frog virus 3 and channel catfish virus by esculentin-2P and ranatuerin-2P, two antimicrobial peptides isolated from frog skin.

    • Reference

    • Virology. 2001 Sep 30;288(2):351-7.

    • Author

    • Chinchar VG, Wang J, Murti G, Carey C, Rollins-Smith L.
  • ·Literature 2

    • Title

    • Peptides with antimicrobial activity from four different families isolated from the skins of the North American frogs Rana luteiventris, Rana berlandieri and Rana pipiens.

    • Reference

    • Eur J Biochem. 2000 Feb;267(3):894-900.

    • Author

    • Goraya J, Wang Y, Li Z, O'Flaherty M, Knoop FC, Platz JE, Conlon JM.