General Information

    • DRAMP ID

    • DRAMP01524

    • Peptide Name

    • Rugosin-RN1 (Frogs, amphibians, animals)

    • Source

    • Rana nigrovittata

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • SIRDKIKTIAIDLAKSAGTGVLKTLICKLDKSC

    • Sequence Length

    • 33

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.19778602]Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC=4.69 µg/ml), Bacillus subtilis (MIC=9.38 µg/ml);
      • Gram-negative bacterium: Escherichia coli ML-35P (MIC=25.00 µg/ml);
      • Yeast: Candida albicans ATCC2002 (MIC=5.63 µg/ml)

    • Hemolytic Activity

      • [Ref:19778602]Show weak hemolytic activity against rabbit red cells

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys27 and Cys33)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bridge between Cys27 and Cys33.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01524 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C153H276N42O46S2

    • Absent Amino Acids

    • EFHMNPQWY

    • Common Amino Acids

    • K

    • Mass

    • 3504.25

    • PI

    • 9.51

    • Basic Residues

    • 7

    • Acidic Residues

    • 3

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +4

    • Boman Index

    • -34.1

    • Hydrophobicity

    • 0.261

    • Aliphatic Index

    • 124.24

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.91

    • Polar Residues

    • 10

DRAMP01524

DRAMP01524 chydropathy plot

Comments Information

    • Function

    • Has weak hemolytic activity gainst rabbit red cells.

Literature Information

  • ·Literature 1

    • Title

    • Peptidomics and genomics analysis of novel antimicrobial peptides from the frog, Rana nigrovittata.

    • Reference

    • Genomics. 2010 Jan;95(1):66-71.

    • Author

    • Ma Y, Liu C, Liu X, Wu J, Yang H, Wang Y, Li J, Yu H, Lai R.