• • DRAMP ID

  • DRAMP01547

• • Peptide Name

  • Nigroain-K2 (Frogs, amphibians, animals)

• • Source

  • Rana nigrovittata (Black-striped frog)

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • SLWETIKNAGKGFILNILDKIRCKVAGGCKT

• • Sequence Length

  • 31

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Not found

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal

• • Target Organism

  • • [Ref.19778602]Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC=11.25 µg/ml), Staphylococcus aureus ATCC43300 (MIC=9.38 µg/ml), Bacillus subtilis (MIC=9.38 µg/ml);
    • Gram-positive bacteria: Escherichia coli ML-35P (MIC=25.00 µg/ml), Pseudomonas aeruginosa PA01 (MIC=25.00 µg/ml), Pseudomonas aeruginosa ATCC27853 (MIC=25.00 µg/ml);
    • Yeast: Candida albicans ATCC2002 (MIC=15.00 µg/ml)

• • Hemolytic Activity

  • • [Ref:19778602]85.52 ± 3.26% hemolytic activity at 50 µg/ml against rabbit red cells

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bridge between Cys23 and Cys29.

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP01547.

DRAMP01547

• • Function

  • Has antibacterial and antifungal activity. Also has hemolytic activity against rabbit red cells.

• ·Literature 1

• • Title

  • Peptidomics and genomics analysis of novel antimicrobial peptides from the frog, Rana nigrovittata.

• • Pubmed ID

  • 19778602

• • Reference

  • Genomics. 2010 Jan;95(1):66-71.

• • Author

  • Ma Y, Liu C, Liu X, Wu J, Yang H, Wang Y, Li J, Yu H, Lai R.