General Information

    • DRAMP ID

    • DRAMP02077

    • Peptide Name

    • Brevinin-1Pb (Frogs, amphibians, animals)

    • Source

    • Rana pipiens (Northern leopard frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLPIIAGIAAKVFPKIFCAISKKC

    • Sequence Length

    • 24

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.10651828]Gram-positive bacterium: Staphylococcus aureus (MIC=5 μM);
      • Gram-negative bacterium: Escherichia coli (MIC=14 μM).
      • Yeast: Candida albicans (MIC=7 μM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization of a C-terminal Cys residue (forming a disulfide bond)

    • Nonterminal Modifications and Unusual Amino Acids

    • There is a disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02077 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C125H204N28O26S2

    • Absent Amino Acids

    • DEHMNQRTWY

    • Common Amino Acids

    • I

    • Mass

    • 2579.29

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +4

    • Boman Index

    • 27.64

    • Hydrophobicity

    • 1.296

    • Aliphatic Index

    • 126.25

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 4

DRAMP02077

DRAMP02077 chydropathy plot

Comments Information

    • Function

    • Antimicrobial activity against the Gram-positive, Gram-negative bacteria and fungi.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Peptides with antimicrobial activity from four different families isolated from the skins of the North American frogs Rana luteiventris, Rana berlandieri and Rana pipiens.

    • Reference

    • Eur J Biochem. 2000 Feb;267(3):894-900.

    • Author

    • Goraya J, Wang Y, Li Z, O'Flaherty M, Knoop FC, Platz JE, Conlon JM.
  • ·Literature 2

    • Title

    • Granular gland transcriptomes in stimulated amphibian skin secretions.

    • Reference

    • Biochem J. 2003 Apr 1;371(Pt 1):125-130.

    • Author

    • Chen T, Farragher S.M, Bjourson A.J, Orr D.F, Rao P, Shaw C.