General Information

    • DRAMP ID

    • DRAMP02274

    • Peptide Name

    • Ranacyclin-E (Frogs, amphibians, animals)

    • Source

    • Rana esculenta (Edible frog) (Pelophylax esculentus)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • SAPRGCWTKSYPPKPCK

    • Sequence Length

    • 17

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.14636071]Gram-positive bacteria: Bacillus megaterium Bm11 (MIC=3 µM), Staphylococcus lentus (MIC=7 µM), Micrococcus luteus (MIC=5 µM);
      • Gram-negative bacteria: Yersinia pseudotuberculosis YP III (MIC=9 µM), Pseudomonas syringae pv tabaci (MIC=80 µM);
      • Fungi: Candida tropicalis (MIC=7.4 µM), C. guillermondii (MIC=3.4 µM), Phytophthora nicotianae spores (MIC=32 µM).

    • Hemolytic Activity

      • [Ref.14636071] It has 55% hemolytic activity at 100 μM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys6 and Cys16.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02274 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP02274.

Physicochemical Information

    • Formula

    • C85H132N24O22S2

    • Absent Amino Acids

    • DEFHILMNQV

    • Common Amino Acids

    • P

    • Mass

    • 1906.25

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 2

    • Net Charge

    • +4

    • Boman Index

    • -33.44

    • Hydrophobicity

    • -1.218

    • Aliphatic Index

    • 5.88

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 7115

    • Absorbance 280nm

    • 444.69

    • Polar Residues

    • 7

DRAMP02274

Comments Information

    • Function

    • Has antibacterial activity. The mature peptide inserts into the hydrophobic core of the bacterial cell membrane and increases permeability without disrupting membrane integrity. Probably binds to the outer membrane surface before aggregating to form transmembrane pores. Has hemolytic activity. Probably binds to the outer membrane surface before aggregating to form transmembrane pores.

    • Tissue specificity

    • Expressed by the skin granular glands.

Literature Information

  • ·Literature 1

    • Title

    • Ranacyclins, a new family of short cyclic antimicrobial peptides: biological function, mode of action, and parameters involved in target specificity.

    • Reference

    • Biochemistry. 2003 Dec 2;42(47):14023-14035.

    • Author

    • Mangoni ML, Papo N, Mignogna G, Andreu D, Shai Y, Barra D, Simmaco M.