• • DRAMP ID

  • DRAMP03089

• • Peptide Name

  • Drosophila cecropin-A1/A2 (Insects, animals)

• • Source

  • Drosophila melanogaster (Fruit fly)

• • Family

  • Belongs to the cecropin family

• • Gene

  • CecA1 AND CecA2

• • Sequence

  • GWLKKIGKKIERVGQHTRDATIQGLGIAQQAANVAATAR

• • Sequence Length

  • 39

• • UniProt Entry

  • P14954,B3DN02,B3DN08,O16815,O16816,O16817,O16818,O16819,O16820

• • Protein Existence

  • Transcript level

• • SMILES

  • CC[C@@H](C)[C@H](NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)CN)[C@H](C)CC)[C@H](C)CC)C(C)C)[C@@H](C)O)[C@@H](C)O)[C@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(=N)N)C(N)=O)[C@@H](C)O)C(C)C

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • Gram-negative bacteria:

      Target Organism	Activity
      Escherichia coli D21	MIC=0.3 µM
      Enterobacter cloacae β12	MIC=0.4 µM
      Pseudomonas aeruginosa OT97	MIC=2.2 µM
      Serratia marcescens Db11 (MIC=93 µM);	-

    • Gram-positive bacteria:

      Target Organism	Activity
      Bacillus megatherium Bm11	MIC=2.6 µM
      Bacillus subtilis Bsll	MIC=16 µM
      Micrococcus luteus MI11	MIC=8.5 µM

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • Not included yet

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • Free

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • PDB ID

• Predicted Structure

• There is no predicted structure for DRAMP03089.

DRAMP03089

• • Function

  • Cecropins have lytic and antibacterial activity against several Gram-positive and Gram-negative bacteria.

• • Tissue specificity

  • Strongly expressed in larval, pupal and adult fat body and hemocytes after injection of bacteria. Maximal expression in the adult involves fat body cells of the head, thorax and abdomen.

• • Induction

  • Induced as part of the humoral response to a bacterial invasion. Transcripts appear within one hour after injection of bacteria into the hemocoel, reach a maximum after 2-6 hours and have almost disappeared after 24 hours. Similar response is seen when flies ingest bacteria present in their food.

• • PTM

  • C-terminal amidation (By similarity).

• ·Literature 1

• • Title

  • The cecropin locus in Drosophila; a compact gene cluster involved in the response to infection.

• • Pubmed ID

  • 2104802

• • Reference

  • EMBO J. 1990 Jan;9(1):217-224.

• • Author

  • Kylsten P, Samakovlis C, Hultmark D.

• ·Literature 2

• • Title

  • The immune response in Drosophila: pattern of cecropin expression and biological activity.

• • Pubmed ID

  • 2390977

• • Reference

  • EMBO J. 1990 Sep;9(9):2969-2976.

• • Author

  • Samakovlis C, Kimbrell DA, Kylsten P, Engström A, Hultmark D.

• ·Literature 3

• • Title

• • Pubmed ID

  • 9725836

• • Reference

• • Author