General Information

    • DRAMP ID

    • DRAMP03593

    • Peptide Name

    • Neutrophil defensin 3 (Defensin, alpha 3; HNP-3, HP-3, HP3; Human, mammals, animals)

    • Source

    • Homo sapiens (Human)

    • Family

    • Belongs to the alpha-defensin family

    • Gene

    • DEFA3

    • Sequence

    • DCYCRIPACIAGERRYGTCIYQGRLWAFCC

    • Sequence Length

    • 30

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiviral(SARS-CoV-2)

    • Target Organism

      • [Ref.34206990]Virus:SARS-CoV-2:inhibition of infection in HEK293T-hACE2 cells(approximately 50% inbibition at 1 μg/mL (290 nM)).
      • Gram-negative bacteria: Escherichia coli ATCC 8739 (vLD50=6.2±0.9 µg/ml), E. coli ATCC 25922 (vLD50=5.9±2.1 µg/ml), Enterobacter aerogenes ATCC 13048 (vLD50=41±9.2 µg/ml);
      • Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (vLD50=13±2.1 µg/ml), S. aureus ATCC 29213 (vLD50=2.2±0.4 µg/ml), Bacillus cereus ATCC 10876 (vLD50=0.37±0.08 µg/ml).(Ref.2)
      • NOTE: vLD50, virtual lethal doses (vLDs), equivalent to conventional 50% lethal doses (LD50s).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization(Cys2 and Cys30).

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys2 and Cys30,Cys4 and Cys19,Cys9 and Cys29.

    • Stereochemistry

    • L

    • Structure

    • Beta strand

    • Structure Description

    • Compared to HNP-2, HNP-3 contains one additional residue (Asp) at the N-terminus. The 3D structures remain the same.

    • Helical Wheel Diagram

    • DRAMP03593 helical wheel diagram
  • 1DFN-> 

    • Predicted Structure

    • There is no predicted structure for DRAMP03593.

Physicochemical Information

    • Formula

    • C151H228N44O40S6

    • Absent Amino Acids

    • HKMNSV

    • Common Amino Acids

    • C

    • Mass

    • 3492.1

    • PI

    • 8.33

    • Basic Residues

    • 4

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +2

    • Boman Index

    • -42.82

    • Hydrophobicity

    • 0.123

    • Aliphatic Index

    • 62

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 10345

    • Absorbance 280nm

    • 356.72

    • Polar Residues

    • 13

DRAMP03593

DRAMP03593 chydropathy plot

Comments Information

    • Function

    • Defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.

    • PTM

    • Contains three disulfide bonds 2-30; 4-19; 9-29.

Literature Information

  • ·Literature 1

    • Title

    • Human Defensins Inhibit SARS-CoV-2 Infection by Blocking Viral Entry.

    • Reference

    • Viruses. 2021 Jun 26;13(7):1246.

    • Author

    • Xu C, Wang A, Marin M, Honnen W, Ramasamy S, Porter E, Subbian S, Pinter A, Melikyan GB, Lu W, Chang TL.
  • ·Literature 2

    • Title

    • Primary structures of three human neutrophil defensins.

    • Reference

    • J Clin Invest. 1985 Oct;76(4):1436-1439.

    • Author

    • Selsted ME, Harwig SS, Ganz T, Schilling JW, Lehrer RI.
  • ·Literature 3

    • Title

    • Antibacterial activity and specificity of the six human {alpha}-defensins.

    • Reference

    • Antimicrob Agents Chemother. 2005 Jan;49(1):269-275.

    • Author

    • Ericksen B, Wu Z, Lu W, Lehrer RI.