• • DRAMP ID

  • DRAMP03596

• • Peptide Name

  • Human defensin-6 (HD-6; Defensin, alpha 6; Human, mammals, animals)

• • Source

  • Homo sapiens (Human)

• • Family

  • Belongs to the alpha-defensin family

• • Gene

  • DEFA6

• • Sequence

  • AFTCHCRRSCYSTEYSYGTCTVMGINHRFCCL

• • Sequence Length

  • 32

• • UniProt Entry

  • Q01524

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antiviral(SARS-CoV-2)

• • Target Organism

  • • [Ref.34206990]Virus:SARS-CoV-2:inhibition of infection in HEK293T-hACE2 cells(HD6 only blocked SARS-CoV-2 infection at the highest concentration tested (50 μg/mL, 13 μM)).
    • Gram-negative bacteria: Escherichia coli ATCC 8739 (vLD50>256 µg/ml), E. coli ATCC 25922 (vLD50=103±14 µg/ml), Enterobacter aerogenes ATCC 13048 (vLD50=156±11 µg/ml);
    • Gram-positive bacteria: Staphylococcus aureus ATCC 25923 (vLD50>256 µg/ml), S. aureus ATCC 29213 (vLD50>256 µg/ml), Bacillus cereus ATCC 10876 (vLD50=25±13 µg/ml).(Ref.3)
    • NOTE: vLD50, virtual lethal doses (vLDs), equivalent to conventional 50% lethal doses (LD50s).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • [Ref.34206990]No cytotoxicity on HEK293T cells up to 50 μg/mL.

• • Binding Target

  • Not found

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • Disulfide bonds between Cys4 and Cys31,Cys6 and Cys20,Cys10 and Cys30.

• • Stereochemistry

  • L

• • Structure

  • Beta strand (3 strands; 19 residues)

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 1ZMQ,3QTE resolved by X-ray.
• 1ZMQ-> 

• Predicted Structure

• There is no predicted structure for DRAMP03596.

DRAMP03596

• • Function

  • Has very low antimicrobial activity against Gram-negative and Gram-positive bacteria. May protect cells against infection with HIV-1.

• • Subunit structure

  • Homodimer.

• • Tissue specificity

  • Paneth cells of the small intestine.

• • PTM

  • Contains three disulfide bonds.

• ·Literature 1

• • Title

  • Human Defensins Inhibit SARS-CoV-2 Infection by Blocking Viral Entry.

• • Pubmed ID

  • 34206990

• • Reference

  • Viruses. 2021 Jun 26;13(7):1246.

• • Author

  • Xu C, Wang A, Marin M, Honnen W, Ramasamy S, Porter E, Subbian S, Pinter A, Melikyan GB, Lu W, Chang TL.

• ·Literature 2

• • Title

  • Defensin-6 mRNA in human Paneth cells: implications for antimicrobial peptides in host defense of the human bowel.

• • Pubmed ID

  • 8417977

• • Reference

  • FEBS Lett. 1993; 315:187-192.

• • Author

  • Jones DE, Bevins CL.

• ·Literature 3

• • Title

  • Crystal structures of human alpha-defensins HNP4, HD5, and HD6.

• • Pubmed ID

  • 17088326

• • Reference

  • Protein Sci. 2006 Dec;15(12):2749-2760.

• • Author

  • Szyk A, Wu Z, Tucker K, Yang D, Lu W, Lubkowski J.

• ·Literature 4

• • Title

  • Antibacterial activity and specificity of the six human {alpha}-defensins.

• • Pubmed ID

  • 15616305

• • Reference

  • Antimicrob Agents Chemother. 2005 Jan;49(1):269-275.

• • Author

  • Ericksen B, Wu Z, Lu W, Lehrer RI.