General Information

    • DRAMP ID

    • DRAMP20776

    • Peptide Name

    • HaA4 (beetles,insects,animals)

    • Source

    • Harmonia axyridis (Multicolored Asian lady beetle)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • IGGYCSWLRL

    • Sequence Length

    • 10

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.23124352] Gram-negative bacteria:
        Target OrganismActivity
        Escherichia coli KACC 13821MIC=32 microM
        Klebsiella pneumoniae KACC 11402MIC<64 microM
        Pseudomonas aeruginosa KACC 10259MIC<64 microM
        Pseudomonas syringae KACC 10659 (MIC=2 microM)；-
      • Gram-positive bacteria:
        Target OrganismActivity
        Staphylococcus aureus KACC 13257MIC<64 microM
        MR Staphylococcus aureus CCARM 3001MIC=32 microM
        Enterococcus faecalis KACC 13807MIC=16 microM
        Enterococcus hirae KACC 16328MIC=32 microM
        Staphylococcus epidermidis KACC 13234MIC=8 microM
        Micrococcus luteus KACC 10488MIC = 4 microM
        Streptococcus pyogenes KACC 11956MIC=32 microM

    • Hemolytic Activity

      • [Ref.23124352] In the hemolytic assay, HaA4 peptide evidenced no hemolytic activity against mouse red blood cells, even at high concentrations, and thus suggesting that the peptide may not be detrimental to eukaryotic cells.

    • Cytotoxicity

      • [Ref.23124352] The cytotoxicity is not found in a dose dependent manner.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP20776 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP20776.

Physicochemical Information

    • Formula

    • C54H82N14O13S

    • Absent Amino Acids

    • ADEFHKMNPQTV

    • Common Amino Acids

    • GL

    • Mass

    • 1167.39

    • PI

    • 8.22

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +1

    • Boman Index

    • 179

    • Hydrophobicity

    • 0.63

    • Aliphatic Index

    • 117

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 6990

    • Absorbance 280nm

    • 776.67

    • Polar Residues

    • 5

DRAMP20776

Comments Information

    • Function

    • Antibacterial activity against the Gram-negative and Gram-positive bacteria. HaA4 is proved to have more potent activity against Staphylococcus epidermidis, M. luteus, and Pseudomonas syringae.

Literature Information

  • ·Literature 1

    • Title

    • Characterization and cDNA cloning of a defensin-like peptide, harmoniasin, from Harmonia axyridis.

    • Reference

    • J Microbiol Biotechnol. 2012 Nov;22(11):1588-90.

    • Author

    • Kim IW, Lee JH, Park HY, Kwon YN, Yun EY, Nam SH, Kim SR, Ahn MY, Hwang JS.