General Information

    • DRAMP ID

    • DRAMP21086

    • Peptide Name

    • PR-FO (Derived from PRW4)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RFRRLRWKTRWRLKKIRFGRFLRKIRRFRPK

    • Sequence Length

    • 31

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.25818457] Gram-positive bacteria:
        Target OrganismActivity
        Staphylococcus aureus 25923MIC=8.6 mg/L
        Staphylococcus aureus 29213MIC=4.3 mg/L
        Staphylococcus epidermidis 12228MIC=2.2 mg/L
        Enterococcus faecalis 29212MIC=1.1 mg/L
        Bacillus subtilis 11060(MIC=2.2 mg/L);-
      • Gram-negative bacteria:
        Target OrganismActivity
        Escherichia coli 078MIC=2.2 mg/L
        Escherichia coli K88MIC=4.3 mg/L
        Escherichia coli ER2566MIC=2.2 mg/L
        Escherichia coli 44102MIC=8.6 mg/L
        Escherichia coli 1515MIC=2.2 mg/L
        Escherichia coli 25922MIC=2.2 mg/L
        Escherichia coli UB1005MIC=4.3 mg/L
        Salmonella typhimurium 7731MIC=0.5 mg/L
        Salmonella typhimurium 1402MIC=8.6 mg/L
        Pseudomonas aeruginosa 21625MIC=8.6 mg/L
        Pseudomonas aeruginosa 21630MIC=0.5 mg/L
        Pseudomonas aeruginosa 10419MIC=4.3 mg/L
        Pseudomonas aeruginosa 27853MIC=8.6 mg/L
        Salmonella pullorum 7913MIC=8.6 mg/L

    • Hemolytic Activity

      • [Ref.25818457] 0% hemolysis at 9.2 mg/L , 1% hemolysis at 18.4 mg/L , 4% hemolysis at 36.8mg/L , 5% hemolysis at 73.6 mg/L , 10% hemolysis at 147.1 mg/L , 13% hemolysis at 294.3 mg/L against human red blood cells

    • Cytotoxicity

      • [Ref.25818457] The cell viability of PR-FO is 93%, 89%, 84%, 79%, 74%, 69%, 67%, 64% and 62% at peptide concentrations of 1.1, 2.3, 4.6, 9.2, 18.4, 36.8, 73.6, 147.1 and 294.3 μg/ml.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil, alpha helix, beta Sheet

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP21086 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21086.

Physicochemical Information

    • Formula

    • C201H334N74O33

    • Absent Amino Acids

    • ACDEHMNQSVY

    • Common Amino Acids

    • R

    • Mass

    • 4315.34

    • PI

    • 13.04

    • Basic Residues

    • 17

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +17

    • Boman Index

    • -16724

    • Hydrophobicity

    • -1.497

    • Aliphatic Index

    • 62.9

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 11000

    • Absorbance 280nm

    • 366.67

    • Polar Residues

    • 2

DRAMP21086

DRAMP21086 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria

Literature Information

  • ·Literature 1

    • Title

    • Characterization of cell selectivity, physiological stability and endotoxin neutralization capabilities of a-helix-based peptide amphiphiles

    • Reference

    • Biomaterials. 2015 Jun;52:517-30. doi: 10.1016/j.biomaterials.2015.02.063.

    • Author

    • Zhi Ma, Dandan Wei, Ping Yan, Xin Zhu, Anshan Shan , Zhongpeng Bi