General Information

    • DRAMP ID

    • DRAMP00033

    • Peptide Name

    • Lantibiotic epilancin 15X (Bacteriocin)

    • Source

    • Staphylococcus epidermidis 15X154 (Gram-positive bacteria)

    • Family

    • Belongs to the type A lantibiotic family (Class I bacteriocin)

    • Gene

    • Not found

    • Sequence

    • ASIVKTTIKASKKLCRGFTLTCGCHFTGKK

    • Sequence Length

    • 30

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: resistant bacterial strains Staphylococcus aureus (MRSA) and VRE.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Non helix or strand structure

    • Structure Description

    • The molecule contains ten post-translationally modified amino acids, three lanthionine ring structures and a hydroxy-propionyl N-terminal moiety.

    • Helical Wheel Diagram

    • DRAMP00033 helical wheel diagram

    • PDB ID

    • 1W9N resolved by NMR.
  • 1W9N-> 

    • Predicted Structure

    • There is no predicted structure for DRAMP00033.

Physicochemical Information

    • Formula

    • C142H243N41O38S3

    • Absent Amino Acids

    • DEMNPQWY

    • Common Amino Acids

    • K

    • Mass

    • 3228.92

    • PI

    • 10.07

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +8

    • Boman Index

    • -32.57

    • Hydrophobicity

    • 0.003

    • Aliphatic Index

    • 68.33

    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 4.31

    • Polar Residues

    • 13

DRAMP00033

DRAMP00033 chydropathy plot

Comments Information

    • Function

    • Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria

    • PTM

    • There are one Lactic acid (S1), one 2,3-didehydroalanine (S3) and three 2,3-dehydrobutyrines (T7; T8; T28); also one Lanthionine (S12-C16) and two Beta-methyllanthionines (T20-C23; T22-C25).

Literature Information

  • ·Literature 1

    • Title

    • Isolation and structural characterization of epilancin 15X, a novel lantibiotic from a clinical strain of Staphylococcus epidermidis.

    • Reference

    • FEBS Lett. 2005 Mar 28;579(9):1917-1922.

    • Author

    • Ekkelenkamp MB, Hanssen M, Danny Hsu ST, de Jong A, Milatovic D, Verhoef J, van Nuland NA.