General Information

    • DRAMP ID

    • DRAMP00186

    • Peptide Name

    • LSEI_2163 (m2163; Bacteriocin)

    • Source

    • Lactobacillus casei ATCC 334 (Gram-positive bacteria)

    • Family

    • Belongs to the class IId bacteriocin

    • Gene

    • Not found

    • Sequence

    • KRKCPKTPFDNTPGAWFAHLILGC

    • Sequence Length

    • 24

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • Gram-negative bacteria: Lactobacillus casei ATCC 393 (++), L. casei ATCC 27139 (++), Lactobacillus rhamnosus ATCC 21052 (+++++), L. rhamnosus ATCC 14957 (+), Lactobacillus plantarum ATCC 8014 (+), Listeria innocua ATCC 33091 (++), L. innocua CIP 78.44 (++), Listeria monocytogens ATCC 19112 (+++), Listeria welshimeri ATCC 43551 (++), Listeria seeligeri CIP79.46 (++++).
      • Note: + indistinct inhibit zone with diameter 8-12 mm, ++ indistinct inhibit zone with diameter ≥12 mm, +++ clear inhibit zone with diameter 12-16 mm, ++++ clear inhibit zone with diameter 16-20 mm, and +++++ clear inhibit zone with diameter ≥20 mm.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00186 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00186.

Physicochemical Information

    • Formula

    • C124H190N34O30S2

    • Absent Amino Acids

    • EMQSVY

    • Common Amino Acids

    • KP

    • Mass

    • 2701.2

    • PI

    • 9.39

    • Basic Residues

    • 5

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +4

    • Boman Index

    • -25.62

    • Hydrophobicity

    • -0.333

    • Aliphatic Index

    • 57.08

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 5625

    • Absorbance 280nm

    • 244.57

    • Polar Residues

    • 7

DRAMP00186

DRAMP00186 chydropathy plot

Comments Information

    • Function

    • mature LSEI_2163 peptide was a class IId bacteriocin that exhibited antimicrobial activity against some lactobacilli and several Listeria species. The activities of both peptides tolerat 121°C for 30 min but not treatment with proteinase K or trypsin.

Literature Information

  • ·Literature 1

    • Title

    • Characterization of putative class II bacteriocins identified from a non-bacteriocin-producing strain Lactobacillus casei ATCC 334.

    • Reference

    • Appl Microbiol Biotechnol. 2012 Jun 12.

    • Author

    • Kuo YC, Liu CF, Lin JF, Li AC, Lo TC, Lin TH.