General Information

    • DRAMP ID

    • DRAMP00267

    • Peptide Name

    • Antifungal lectin AMML (AMML; Plant defensin)

    • Source

    • Astragalus mongholicus (Huang qi) (Astragalus membranaceus varmongholicus)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • ESGINLQGDATLANN

    • Sequence Length

    • 15

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal

    • Target Organism

      • Fungi: Botrytis cinerea (IC50=1.2 µM), Drechslera turcia, Fusarium oxysporum and Colletorichum sp.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Galactose and Glucose

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00267 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00267.

Physicochemical Information

    • Formula

    • C61H101N19O26

    • Absent Amino Acids

    • CFHKMPRVWY

    • Common Amino Acids

    • N

    • Mass

    • 1516.58

    • PI

    • 3.67

    • Basic Residues

    • 0

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 5

    • Net Charge

    • -2

    • Boman Index

    • -26.7

    • Hydrophobicity

    • -0.507

    • Aliphatic Index

    • 91.33

    • Half Life

      • Mammalian:1 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 7

DRAMP00267

Comments Information

    • Function

    • Lectin that binds strongly to galactose, lactose, D-raffinose, L-rhamnose and cellobiose, and less strongly to D-glucose, D-xylose and L-arabinose. Does not bind D-galacturonic acid, D-fructose, D-mannose, D-ribose, L-sorbose, maltose and sucrose. Has hemagglutinating activity towards human type O and rabbit erythrocytes. Has strong antifungal activity against B. cinera, and weaker antifungal activity against D. turcia, F. oxysporum and Colletorichum sp. but not against Rhizoctonia solani and Mycosphaerella arachidicola.

    • Miscellaneous

    • Hemagglutinating activity stable when incubated at 65 degrees Celsius for 30 minutes. Hemagglutinating activity is reduced by 50% when incubated at 75 degrees Celsius for 30 minutes and is abolished by incubation at 85 degrees Celsius for 30 minutes. Hemagglutinating activity is stable between pH 4.5 and 7.5, but is completely inhibited by incubation below pH 3.0 or above pH 10.

Literature Information

  • ·Literature 1

    • Title

    • A novel homodimeric lectin from Astragalus mongholicus with antifungal activity.

    • Reference

    • Arch Biochem Biophys. 2005 Oct 1;442(1):72-81.

    • Author

    • Yan Q, Jiang Z, Yang S, Deng W, Han L.