General Information

    • DRAMP ID

    • DRAMP00268

    • Peptide Name

    • Antifungal protein Pr-2 (Pr-2; Plant defensin)

    • Source

    • Cucurbita maxima (Pumpkin) (Winter squash)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • QGIGVGDNDGKRGKR

    • Sequence Length

    • 15

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal

    • Target Organism

      • [Ref.19807165]Fungi: Botrytis cinerea (EC50=20 µM), Candida albicans (EC50=20 µM), Colletotrichum coccodes (EC50=10 µM), Fusarium oxysporum (EC50=10 µM), Fusarium solani (EC50=10 µM), Trichoderma harzianum (EC50=20 µM), Trichoderma viride (EC50=20 µM), Aspergillus fumigatus EC50=40 µM), Aspergillus parasiticus (EC50=80 µM), Didymella bryoniae (EC50=40 µM).

    • Hemolytic Activity

      • [Ref:19807165]0% hemolytic activity at 80 µM and 8% hemolytic activity at 160 µM against huamn red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00268 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00268.

Physicochemical Information

    • Formula

    • C62H109N25O22

    • Absent Amino Acids

    • ACEFHLMPSTWY

    • Common Amino Acids

    • G

    • Mass

    • 1556.7

    • PI

    • 9.99

    • Basic Residues

    • 4

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 2

    • Net Charge

    • +2

    • Boman Index

    • -56.9

    • Hydrophobicity

    • -1.607

    • Aliphatic Index

    • 45.33

    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 6

DRAMP00268

Comments Information

    • Function

    • Has strong antifungal activity. Lacks antifungal activity against P.verrucosum var. verrucosum. Lacks antibacterial activity against the Gram-negative bacterium E.coli and against the Gram-positive bacterium S.aureus. Has very low hemolytic activity against human erythrocytes. Increases fungal cell membrane permeability, probably by disrupting the membrane structure.

Literature Information

  • ·Literature 1

    • Title

    • Antifungal mechanism of a novel antifungal protein from Pumpkin rinds against various fungal pathogens.

    • Reference

    • J Agric Food Chem. 2009 Oct 14;57(19):9299-9304.

    • Author

    • Park SC, Kim JY, Lee JK, Hwang I, Cheong H, Nah JW, Hahm KS, Park Y.