General Information
-
DRAMP ID
- DRAMP00295
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Peptide Name
- Soybean toxin 27 kDa chain (SBTX 27 kDa chain; Plant defensin)
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Source
- Glycine max (Soybean) (Glycine hispida)
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Family
- Not found
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Gene
- Not found
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Sequence
- ADPTFGFTPLGLSEKANLQIMKAYD
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Sequence Length
- 25
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UniProt Entry
- P86520
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antifungal
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Target Organism
-
- Fungi: C. sojina.
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Not included yet
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N-terminal Modification
- Not included yet
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C-terminal Modification
- Not included yet
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Nonterminal Modifications and Unusual Amino Acids
- Not included yet
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Stereochemistry
- Not included yet
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Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP00295.
Physicochemical Information
-
Formula
- C124H191N29O38S
Absent Amino Acids
- CHRVW
Common Amino Acids
- AL
Mass
- 2728.11
PI
- 4.56
Basic Residues
- 2
Acidic Residues
- 3
Hydrophobic Residues
- 9
Net Charge
- -1
-
Boman Index
- -20.91
Hydrophobicity
- -0.16
Aliphatic Index
- 74.4
Half Life
-
- Mammalian:4.4 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 1490
Absorbance 280nm
- 62.08
Polar Residues
- 7
DRAMP00295
Comments Information
Function
- Involved in plant defense. Has antifungal activity against C. sojina but not A. niger and F. solani. Does not have urease or hemagglutination activities. Does not inhibit trypsin. Injection into mice produces toxic effects such as dyspnea, tonic-clonic convulsion and death.
Subunit structure Heterodimer of a 27 kDa subunit and a 17 kDa subunit; disulfide-linked.
Tissue specificity
- Expressed in seeds, leaves, roots and stem (at protein level).
Induction
- In seeds, induced by jasmonate.
Post-translational modification
- SBTX is known to be glycosylated but it is not known which one of its two subunits is modified; contains 5% carbohydrates.
Toxic dose
- LD50 is 5.6 mg/kg by intraperitoneal injection into mice.
Miscellaneous
- On the 2D-gel the determined pI of SBTX is
Biophysicochemical properties
- pH dependence (Activity decreases below pH 5.5 and above pH 8.0).
Literature Information
- ·Literature 1
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Title
- SBTX, a new toxic protein distinct from soyatoxin and other toxic soybean [Glycine max] proteins, and its inhibitory effect on Cercospora sojina growth.
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Pubmed ID
- 18328522
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Reference
- Toxicon. 2008 May;51(6):952-963.
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Author
- Vasconcelos IM, Morais JK, Siebra EA, Carlini CR, Sousa DO, Beltramini LM, Melo VM, Oliveira JT.