• DRAMP ID

    • DRAMP00295
    • Peptide Name

    • Soybean toxin 27 kDa chain (SBTX 27 kDa chain; Plant defensin)
    • Source

    • Glycine max (Soybean) (Glycine hispida)
    • Family

    • N/A
    • Gene

    • N/A
    • Sequence

    • ADPTFGFTPLGLSEKANLQIMKAYD
    • Sequence Length

    • 25
    • Evidence code

    • Protein level
    • Biological Activity

    • Antifungal, Antimicrobial
    • Target Organism

      • Fungi: C. sojina.
    • Hemolytic Activity

      • N/A
    • Cytotoxicity

    • Binding Target

    • N/A
    • Linear/Cyclic/Branched

    • N-terminal modification

    • C-terminal modification

    • Other modifications

    • Stereochemistry

    • Structure

    • N/A
    • Structure Description

    • N/A
    • DRAMP00295 helical wheel diagram
    • Formula

    • C124H191N29O38S
    • Absent Amino Acids

    • CHRVW
    • Common Amino Acids

    • AL
    • Mass

    • 3156.39
    • PI

    • 4.37
    • Basic Residues

    • 2
    • Acidic Residues

    • 3
    • Hydrophobic Residues

    • 9
    • Boman Index

    • -20.91
    • Hydrophobicity

    • -16
    • Aliphatic Index

    • 74.4
    • Half Life

      • Mammalian:4.4 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 1490
    • Absorbance 280nm

    • 62.08
    • Polar Residues

    • 7

DRAMP00295

DRAMP00295 chydropathy plot
    • Function

    • Involved in plant defense. Has antifungal activity against C. sojina but not A. niger and F. solani. Does not have urease or hemagglutination activities. Does not inhibit trypsin. Injection into mice produces toxic effects such as dyspnea, tonic-clonic convulsion and death.
    • Subunit structure Heterodimer of a 27 kDa subunit and a 17 kDa subunit; disulfide-linked.

    • Tissue specificity

    • Expressed in seeds, leaves, roots and stem (at protein level).
    • Induction

    • In seeds, induced by jasmonate.
    • Post-translational modification

    • SBTX is known to be glycosylated but it is not known which one of its two subunits is modified; contains 5% carbohydrates.
    • Toxic dose

    • LD50 is 5.6 mg/kg by intraperitoneal injection into mice.
    • Miscellaneous

    • On the 2D-gel the determined pI of SBTX is
    • Biophysicochemical properties

    • pH dependence (Activity decreases below pH 5.5 and above pH 8.0).
  • Literature 1
    • Reference

    • Toxicon. 2008 May;51(6):952-963.
    • Author

    • Vasconcelos IM, Morais JK, Siebra EA, Carlini CR, Sousa DO, Beltramini LM, Melo VM, Oliveira JT.
    • Title

    • SBTX, a new toxic protein distinct from soyatoxin and other toxic soybean [Glycine max] proteins, and its inhibitory effect on Cercospora sojina growth.