General Information

    • DRAMP ID

    • DRAMP00412

    • Peptide Name

    • Defensin-like protein 1 (Br-AFP1; Plant defensin)

    • Source

    • Brassica rapa (Turnip)

    • Family

    • Belongs to the DEFL family

    • Gene

    • Not found

    • Sequence

    • QKLCERPSGTWSGVCGNNNACKNQCIN

    • Sequence Length

    • 27

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal

    • Target Organism

      • Fungi: Alternaria brassicola (IC50=3 µg/ml), Botrytis cinerea(IC50=1.5 µg/ml), Fusarium culmorum (IC50=2 µg/ml), Fusarium oxysporum f.sp. lycopersici (IC50=1.8 µg/ml), Pyricularia oryzae (IC50=0.25 µg/ml), Verticiliium dahliae (IC50=0.8 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00412 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00412.

Physicochemical Information

    • Formula

    • C117H190N40O40S4

    • Absent Amino Acids

    • DFHMY

    • Common Amino Acids

    • N

    • Mass

    • 2925.28

    • PI

    • 8.53

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 5

    • Net Charge

    • +2

    • Boman Index

    • -60.52

    • Hydrophobicity

    • -0.815

    • Aliphatic Index

    • 43.33

    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5750

    • Absorbance 280nm

    • 221.15

    • Polar Residues

    • 15

DRAMP00412

DRAMP00412 chydropathy plot

Comments Information

    • Function

    • Possesses antifungal activity sensitive to inorganic cations.

    • Subunit structure

    • Forms oligomers in its native state.

Literature Information

  • ·Literature 1

    • Title

    • A new family of basic cysteine-rich plant antifungal proteins from Brassicaceae species.

    • Reference

    • FEBS Lett. 1993 Feb 1;316(3):233-240.

    • Author

    • Terras FR, Torrekens S, Van Leuven F, Osborn RW, Vanderleyden J, Cammue BP, Broekaert WF.