General Information

    • DRAMP ID

    • DRAMP00857

    • Peptide Name

    • Kalata-B2 (Plant defensin)

    • Source

    • Oldenlandia affinis

    • Family

    • Belongs to the cyclotide family

    • Gene

    • OAK4

    • Sequence

    • GLPVCGETCFGGTCNTPGCSCTWPICTRD

    • Sequence Length

    • 29

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Insecticidal

    • Target Organism

      • [Ref.17534989]Insects: Helicoverpa armigera

    • Hemolytic Activity

      • [Ref.20564013] HD50=3.3±1.9 μM against Human red blood cells .

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys5 and Cys19,Cys9 and Cys21,Cys14 and Cys26.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00857 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00857.

Physicochemical Information

    • Formula

    • C122H188N34O41S6

    • Absent Amino Acids

    • AHKMQY

    • Common Amino Acids

    • C

    • Mass

    • 2979.4

    • PI

    • 4.37

    • Basic Residues

    • 1

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 5

    • Net Charge

    • -1

    • Boman Index

    • -21.77

    • Hydrophobicity

    • 0.114

    • Aliphatic Index

    • 36.9

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5875

    • Absorbance 280nm

    • 209.82

    • Polar Residues

    • 18

DRAMP00857

DRAMP00857 chydropathy plot

Comments Information

    • Function

    • Probably participates in a plant defense mechanism. Inhibitory effect on the growth and development of Helicoverpa armigera larvae suggests a role for the cyclotides in plant defense. Has hemolytic activity.

    • PTM

    • Kalata-B2 is a cyclic peptide which contains three disulfide bonds 5-19; 9-21; 14-26.

Literature Information

  • ·Literature 1

    • Title

    • Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif.

    • Reference

    • J Mol Biol. 1999 Dec 17;294(5):1327-1336.

    • Author

    • Craik DJ, Daly NL, Bond T, Waine C.
  • ·Literature 2

    • Title

    • Isolation, solution structure, and insecticidal activity of kalata B2, a circular protein with a twist: do Möbius strips exist in nature?

    • Reference

    • Biochemistry. 2005 Jan 25;44(3):851-860.

    • Author

    • Jennings CV, Rosengren KJ, Daly NL, Plan M, Stevens J, Scanlon MJ, Waine C, Norman DG, Anderson MA, Craik DJ.
  • ·Literature 3

    • Title

    • The cyclotide fingerprint in oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides.

    • Reference

    • Chembiochem. 2007 Jun 18;8(9):1001-1011.

    • Author

    • Plan MR, Göransson U, Clark RJ, Daly NL, Colgrave ML, Craik DJ.
  • ·Literature 4

    • Title

    • Structural and biochemical characteristics of the cyclotide kalata B5 from Oldenlandia affinis

    • Reference

    • Biopolymers . 2010;94(5):647-58.

    • Author

    • Manuel R Plan, K Johan Rosengren, Lillian Sando, Norelle L Daly, David J Craik