General Information
-
DRAMP ID
- DRAMP00859
-
Peptide Name
- Kalata-B4 (Plant defensin)
-
Source
- Oldenlandia affinis
-
Family
- Belongs to the cyclotide family
-
Gene
- Not found
-
Sequence
- GLPVCGETCVGGTCNTPGCTCSWPVCTRD
-
Sequence Length
- 29
-
UniProt Entry
- P83938
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Not found
-
Target Organism
- No MICs found in DRAMP database
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Binds to the micelle surface
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- No specific N-terminal
-
C-terminal Modification
- No specific C-terminal
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys5 and Cys19; Cys9 and Cys21; Cys14 and Cys26.
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Stereochemistry
- L
-
Structure
- Beta strand (4 strands; 10 residues)
-
Structure Description
- The charged residues (Glu3 and Arg24), along with the cation-binding site (near Glu3) are segregated on the other side of the molecule and in contact with polar head groups of detergent. The spatial structure of kalata B1 is only slightly changed during incorporation into micelles and represents a distorted triple-stranded beta-sheet cross-linked by a cystine knot. Detailed structural analysis and comparison with other knottins revealed
-
Helical Wheel Diagram
-
PDB ID
- 1ZNU resolved by NMR.
- 1ZNU-> 
-
Predicted Structure
- There is no predicted structure for DRAMP00859.
Physicochemical Information
-
Formula
- C117H186N34O41S6
Absent Amino Acids
- AFHIKMQY
Common Amino Acids
- C
Mass
- 2917.33
PI
- 4.37
Basic Residues
- 1
Acidic Residues
- 2
Hydrophobic Residues
- 5
Net Charge
- -1
-
Boman Index
- -21.59
Hydrophobicity
- 0.152
Aliphatic Index
- 43.45
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5875
Absorbance 280nm
- 209.82
Polar Residues
- 18
DRAMP00859
Comments Information
Function
- Probably participates in a plant defense mechanism.
PTM
- Kalata-B4 is a cyclic peptide which may contain three disulfide bonds 5-19; 9-21; 14-26.
Literature Information
- ·Literature 1
-
Title
- Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif.
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Pubmed ID
- 10600388
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Reference
- J Mol Biol. 1999 Dec 17;294(5):1327-1336.
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Author
- Craik DJ, Daly NL, Bond T, Waine C.
- ·Literature 2
-
Title
- Conformation and mode of membrane interaction in cyclotides. Spatial structure of kalata B1 bound to a dodecylphosphocholine micelle.
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Pubmed ID
- 16817894
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Reference
- FEBS J. 2006 Jun;273(12):2658-2672.
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Author
- Shenkarev ZO, Nadezhdin KD, Sobol VA, Sobol AG, Skjeldal L, Arseniev AS.