General Information

    • DRAMP ID

    • DRAMP00859

    • Peptide Name

    • Kalata-B4 (Plant defensin)

    • Source

    • Oldenlandia affinis

    • Family

    • Belongs to the cyclotide family

    • Gene

    • Not found

    • Sequence

    • GLPVCGETCVGGTCNTPGCTCSWPVCTRD

    • Sequence Length

    • 29

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Not found

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Binds to the micelle surface

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys5 and Cys19; Cys9 and Cys21; Cys14 and Cys26.

    • Stereochemistry

    • L

    • Structure

    • Beta strand (4 strands; 10 residues)

    • Structure Description

    • The charged residues (Glu3 and Arg24), along with the cation-binding site (near Glu3) are segregated on the other side of the molecule and in contact with polar head groups of detergent. The spatial structure of kalata B1 is only slightly changed during incorporation into micelles and represents a distorted triple-stranded beta-sheet cross-linked by a cystine knot. Detailed structural analysis and comparison with other knottins revealed

    • Helical Wheel Diagram

    • DRAMP00859 helical wheel diagram

    • PDB ID

    • 1ZNU resolved by NMR.

    • Predicted Structure

    • There is no predicted structure for DRAMP00859.

Physicochemical Information

    • Formula

    • C117H186N34O41S6

    • Absent Amino Acids

    • AFHIKMQY

    • Common Amino Acids

    • C

    • Mass

    • 2917.33

    • PI

    • 4.37

    • Basic Residues

    • 1

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 5

    • Net Charge

    • -1

    • Boman Index

    • -21.59

    • Hydrophobicity

    • 0.152

    • Aliphatic Index

    • 43.45

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5875

    • Absorbance 280nm

    • 209.82

    • Polar Residues

    • 18

DRAMP00859

DRAMP00859 chydropathy plot

Comments Information

    • Function

    • Probably participates in a plant defense mechanism.

    • PTM

    • Kalata-B4 is a cyclic peptide which may contain three disulfide bonds 5-19; 9-21; 14-26.

Literature Information

  • ·Literature 1

    • Title

    • Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif.

    • Reference

    • J Mol Biol. 1999 Dec 17;294(5):1327-1336.

    • Author

    • Craik DJ, Daly NL, Bond T, Waine C.
  • ·Literature 2

    • Title

    • Conformation and mode of membrane interaction in cyclotides. Spatial structure of kalata B1 bound to a dodecylphosphocholine micelle.

    • Reference

    • FEBS J. 2006 Jun;273(12):2658-2672.

    • Author

    • Shenkarev ZO, Nadezhdin KD, Sobol VA, Sobol AG, Skjeldal L, Arseniev AS.