General Information

    • DRAMP ID

    • DRAMP00859

    • Peptide Name

    • Kalata-B4 (Plant defensin)

    • Source

    • Oldenlandia affinis

    • Family

    • Belongs to the cyclotide family

    • Gene

    • Not found

    • Sequence

    • GLPVCGETCVGGTCNTPGCTCSWPVCTRD

    • Sequence Length

    • 29

    • Protein Existence

    • Protein level

    • SMILES

    • Not available

Activity Information

    • Biological Activity

    • Not found

    • Target Organism

    • No MICs found in DRAMP database

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Binds to the micelle surface

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • No specific N-terminal

    • C-terminal Modification

    • No specific C-terminal

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bonds between Cys5 and Cys19; Cys9 and Cys21; Cys14 and Cys26.

    • Stereochemistry

    • L

    • PDB ID

    • 1ZNU resolved by NMR.

    • Predicted Structure

Physicochemical Information

    • Formula

    • C117H186N34O41S6

    • Absent Amino Acids

    • AFHIKMQY

    • Common Amino Acids

    • C

    • Mass

    • 2917.33

    • PI

    • 4.37

    • Basic Residues

    • 1

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 5

    • Net Charge

    • -1

    • Boman Index

    • -21.59

    • Hydrophobicity

    • 0.152

    • Aliphatic Index

    • 43.45

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5875

    • Absorbance 280nm

    • 209.82

    • Polar Residues

    • 18

DRAMP00859

DRAMP00859 chydropathy plot

Comments Information

    • Function

    • Probably participates in a plant defense mechanism.

    • PTM

    • Kalata-B4 is a cyclic peptide which may contain three disulfide bonds 5-19; 9-21; 14-26.

Literature Information

  • ·Literature 1

    • Title

    • Plant cyclotides: A unique family of cyclic and knotted proteins that defines the cyclic cystine knot structural motif.

    • Reference

    • J Mol Biol. 1999 Dec 17;294(5):1327-1336.

    • Author

    • Craik DJ, Daly NL, Bond T, Waine C.
  • ·Literature 2

    • Title

    • Conformation and mode of membrane interaction in cyclotides. Spatial structure of kalata B1 bound to a dodecylphosphocholine micelle.

    • Reference

    • FEBS J. 2006 Jun;273(12):2658-2672.

    • Author

    • Shenkarev ZO, Nadezhdin KD, Sobol VA, Sobol AG, Skjeldal L, Arseniev AS.