General Information
-
DRAMP ID
- DRAMP00915
-
Peptide Name
- Cyclotide vibi-C (Plant defensin)
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Source
- Viola biflora (Yellow wood violet)
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Family
- Belongs to the cyclotide family
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Gene
- Not found
-
Sequence
- GLPVCGETCAFGSCYTPGCSCSWPVCTRN
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Sequence Length
- 29
-
UniProt Entry
- P85241
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Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Cytotoxic
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Target Organism
- No MICs found in DRAMP database
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
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N-terminal Modification
- No specific N-terminal
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C-terminal Modification
- No specific C-terminal
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Nonterminal Modifications and Unusual Amino Acids
- Disulfide bonds between Cys5 and Cys19,Cys9 and Cys21,Cys14 and Cys26.
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Stereochemistry
- L
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Structure
- Bridge
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Structure Description
- The cyclotides are head-to-tail macrocyclic and they have three disulfides arranged in a cystine knot, in which two disulfide bonds and their connecting backbone segments form an embedded ring that is penetrated by the third disulfide bond. Cyclotides exhibit a broad range of biological effects, including insecticidal, haemolytic, and cytotoxic effects.
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP00915.
Physicochemical Information
-
Formula
- C125H188N34O40S6
Absent Amino Acids
- DHIKMQ
Common Amino Acids
- C
Mass
- 2999.43
PI
- 5.96
Basic Residues
- 1
Acidic Residues
- 1
Hydrophobic Residues
- 6
Net Charge
- 0
-
Boman Index
- -14.86
Hydrophobicity
- 0.248
Aliphatic Index
- 36.9
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 7365
Absorbance 280nm
- 263.04
Polar Residues
- 18
DRAMP00915
![DRAMP00915 chydropathy plot](http://dramp.cpu-bioinfor.org/tmp/all_info_png/pepwindow_DRAMP00915.1.png)
Comments Information
Function
- Probably participates in a plant defense mechanism and shows potent cytotoxic activity.
PTM
- Contains three disulfide bonds 5-19; 9-21; 14-26. This is a cyclic peptide (cross-link between G1-N29).
Literature Information
- ·Literature 1
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Title
- The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity.
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Pubmed ID
- 18191970
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Reference
- Phytochemistry. 2008 Feb;69(4):939-952.
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Author
- Herrmann A, Burman R, Mylne JS, Karlsson G, Gullbo J, Craik DJ, Clark RJ, Göransson U.