General Information

    • DRAMP ID

    • DRAMP00954

    • Peptide Name

    • Viscotoxin-A3 (Plant defensin)

    • Source

    • Viscum album (European mistletoe)

    • Family

    • Belongs to the thionin family

    • Gene

    • THI2.1

    • Sequence

    • KSCCPNTTGRNIYNACRLTGAPRPTCAKLSGCKIISGSTCPSDYPK

    • Sequence Length

    • 46

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal, Cytotoxic

    • Target Organism

      • Fungi: Fusarium solani, Sclerotinia sclerotiorum, Phytophtora infestans.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrance

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Combine helix and strand structure

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00954 helical wheel diagram

    • PDB ID

    • 1OKH resolved by X-ray.

    • Predicted Structure

    • There is no predicted structure for DRAMP00954.

Physicochemical Information

    • Formula

    • C201H334N62O64S6

    • Absent Amino Acids

    • EFHMQVW

    • Common Amino Acids

    • C

    • Mass

    • 4835.6

    • PI

    • 9.3

    • Basic Residues

    • 7

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +6

    • Boman Index

    • -84.26

    • Hydrophobicity

    • -0.463

    • Aliphatic Index

    • 48.91

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 3355

    • Absorbance 280nm

    • 74.56

    • Polar Residues

    • 25

DRAMP00954

DRAMP00954 chydropathy plot

Comments Information

    • Function

    • Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane.

    • PTM

    • Contains three disulfide bonds 3-40; 4-32; 16-26.

Literature Information

  • ·Literature 1

    • Title

    • Isolation and characterization of cDNAs encoding viscotoxins of mistletoe (Viscum album).

    • Reference

    • Eur J Biochem. 1991 Jun 15;198(3):549-553.

    • Author

    • Schrader G, Apel K.
  • ·Literature 2

    • Title

    • The disulfide bonds of viscotoxin A3 from the European mistletoe (Viscum album L., Loranthaceae).

    • Reference

    • Acta Chem Scand. 1971;25(6):2048-2054.

    • Author

    • Samuelsson G, Pettersson B.
  • ·Literature 3

    • Title

    • NMR structural determination of viscotoxin A3 from Viscum album L.

    • Reference

    • Biochem J. 2000 Sep 1;350 Pt 2:569-577.

    • Author

    • Romagnoli S, Ugolini R, Fogolari F, Schaller G, Urech K, Giannattasio M, Ragona L, Molinari H.