General Information
-
DRAMP ID
- DRAMP00954
-
Peptide Name
- Viscotoxin-A3 (Plant defensin)
-
Source
- Viscum album (European mistletoe)
-
Family
- Belongs to the thionin family
-
Gene
- THI2.1
-
Sequence
- KSCCPNTTGRNIYNACRLTGAPRPTCAKLSGCKIISGSTCPSDYPK
-
Sequence Length
- 46
-
UniProt Entry
- P01538
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antifungal, Cytotoxic
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Target Organism
-
- Fungi: Fusarium solani, Sclerotinia sclerotiorum, Phytophtora infestans.
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Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
-
Binding Target
- Cell membrance
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Combine helix and strand structure
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- 1OKH resolved by X-ray.
- 1OKH-> 
-
Predicted Structure
- There is no predicted structure for DRAMP00954.
Physicochemical Information
-
Formula
- C201H334N62O64S6
Absent Amino Acids
- EFHMQVW
Common Amino Acids
- C
Mass
- 4835.6
PI
- 9.3
Basic Residues
- 7
Acidic Residues
- 1
Hydrophobic Residues
- 8
Net Charge
- +6
-
Boman Index
- -84.26
Hydrophobicity
- -0.463
Aliphatic Index
- 48.91
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 3355
Absorbance 280nm
- 74.56
Polar Residues
- 25
DRAMP00954
Comments Information
Function
- Thionins are small plant proteins which are toxic to animal cells. They seem to exert their toxic effect at the level of the cell membrane.
PTM
- Contains three disulfide bonds 3-40; 4-32; 16-26.
Literature Information
- ·Literature 1
-
Title
- Isolation and characterization of cDNAs encoding viscotoxins of mistletoe (Viscum album).
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Pubmed ID
- 1710983
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Reference
- Eur J Biochem. 1991 Jun 15;198(3):549-553.
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Author
- Schrader G, Apel K.
- ·Literature 2
-
Title
- The disulfide bonds of viscotoxin A3 from the European mistletoe (Viscum album L., Loranthaceae).
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Pubmed ID
- 4941626
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Reference
- Acta Chem Scand. 1971;25(6):2048-2054.
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Author
- Samuelsson G, Pettersson B.
- ·Literature 3
-
Title
- NMR structural determination of viscotoxin A3 from Viscum album L.
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Pubmed ID
- 10947973
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Reference
- Biochem J. 2000 Sep 1;350 Pt 2:569-577.
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Author
- Romagnoli S, Ugolini R, Fogolari F, Schaller G, Urech K, Giannattasio M, Ragona L, Molinari H.