• DRAMP ID

    • DRAMP00976
    • Peptide Name

    • Antimicrobial peptide 1 (AC-AMP1; Plant defensin)
    • Source

    • Amaranthus caudatus (Love-lies-bleeding) (Inca-wheat)
    • Family

    • N/A
    • Gene

    • N/A
    • Sequence

    • VGECVRGRCPSGMCCSQFGYCGKGPKYCG
    • Sequence Length

    • 29
    • Evidence code

    • Protein level
    • Biological Activity

    • Antibacterial, Antifungal, Anti-Gram+, Antimicrobial
    • Target Organism

      • Gram-positive bacteria: Bacillus megaterium (IC50=40 µg/ml), Sarcina lutea (IC50=250 µg/ml).
      • Fungi: Alternaria brassicola (IC50=7 µg/ml), Ascochyta pisi (IC50=8 µg/ml), Botrytis cinera (IC50=10 µg/ml), Colletotrichum lindemuthianum (IC50=8 µg/ml), Fusarium culmorum (IC50=2 µg/ml), Trichoderma hamatum (IC50=7 µg/ml), Verticillium dahliae (IC50=6 µg/ml).
    • Hemolytic Activity

      • N/A
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Chitin-binding
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Other Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Bridge
    • Structure Description

    • N/A
    • DRAMP00976 helical wheel diagram
    • Formula

    • C124H194N38O37S7
    • Absent Amino Acids

    • ADHILNTW
    • Common Amino Acids

    • G
    • Mass

    • 3533.17
    • PI

    • 8.38
    • Basic Residues

    • 4
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 3
    • Boman Index

    • -32.7
    • Hydrophobicity

    • -20.34
    • Aliphatic Index

    • 20
    • Half Life

      • Mammalian:100 hour
      • Yeast:>20 hour
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 3355
    • Absorbance 280nm

    • 119.82
    • Polar Residues

    • 17

DRAMP00976

DRAMP00976 chydropathy plot
    • Function

    • Chitin-binding protein with a defensive Function
    • Miscellaneous

    • Its chitin-binding activity is strongly inhibited by divalent cations.
    • PTM

    • Contains three disulfide bonds 4-15; 9-21; 14-28.
  • Literature 1
    • Title

    • Antimicrobial peptides from Amaranthus caudatus seeds with sequence homology to the cysteine/glycine-rich domain of chitin-binding proteins.
    • Reference

    • Biochemistry. 1992 May 5;31(17):4308-4314.
    • Author

    • Broekaert W.F, Marien W, Terras F.R.G, de Bolle M.F.C, Proost P, van Damme J, Dillen L, Claeys M, Rees S.B, Vanderleyden J, Cammue B.P.A.
  • Literature 2
    • Title

    • Location of the three disulfide bonds in an antimicrobial peptide from Amaranthus caudatus using mass spectrometry.
    • Reference

    • J Pept Res. 1997 Apr;49(4):336-340.
    • Author

    • el Bouyoussfi M, Laus G, Verheyden P, Wyns L, Tourwe D, Van Binst G.