General Information
-
DRAMP ID
- DRAMP00985
-
Peptide Name
- Pn-AMP1 (PnAMP1; Plant defensin)
-
Source
- Ipomoea nil (Japanese morning glory) (Pharbitis nil)
-
Family
- Belongs to the hevein-like family
-
Gene
- Not found
-
Sequence
- QQCGRQASGRLCGNRLCCSQWGYCGSTASYCGAGCQSQCRS
-
Sequence Length
- 41
-
UniProt Entry
- P81591
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Antifungal
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Target Organism
-
- Gram-positive Bacterium: Bacillus subtilis (IC50=38 µg/ml).
- Fungi (Medium A, Medium A+): Botrytis cinerea (IC50=16, 150 µg/ml), Colletotrichum langenarium (IC50=10, >200 µg/ml), Sclerotinia sclerotiorum (IC50=11, >200 µg/ml), Fusarium oxysporum (IC50=10, >200 µg/ml), Rhizoctonia solani (IC50=26, >200 µg/ml), Phytophthora capsici (IC50=5, 130 µg/ml), Phytophthora parasitica (IC50=3, 90 µg/ml), Saccharomyces cerevisiae (IC50=14, >200 µg/ml).
- NOTE! Medium A+ = Medium A supplemented with 5 mM CaCl2.
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
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Cytotoxicity
-
- Not included yet
-
Binding Target
- Chitin-binding
Structure Information
-
Linear/Cyclic
- Not included yet
-
N-terminal Modification
- Not included yet
-
C-terminal Modification
- Not included yet
-
Nonterminal Modifications and Unusual Amino Acids
- Not included yet
-
Stereochemistry
- Not included yet
-
Structure
- Bridge
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP00985.
Physicochemical Information
-
Formula
- C168H267N61O58S8
Absent Amino Acids
- DEFHIKMPV
Common Amino Acids
- C
Mass
- 4325.82
PI
- 8.76
Basic Residues
- 4
Acidic Residues
- 0
Hydrophobic Residues
- 6
Net Charge
- +4
-
Boman Index
- -88.39
Hydrophobicity
- -0.52
Aliphatic Index
- 26.34
Half Life
-
- Mammalian:0.8 hour
- Yeast:10 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 8980
Absorbance 280nm
- 224.5
Polar Residues
- 25
DRAMP00985
Comments Information
Miscellaneous
- Its antimicrobial activity is strongly inhibited by divalent cations.
PTM
- Contains four disulfide bonds 3-18; 12-24; 17-31; 35-39, and Pyrrolidone carboxylic acid at position 1.
Literature Information
- ·Literature 1
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Title
- Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity.
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Pubmed ID
- 9507071
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Reference
- Biochim Biophys Acta. 1998 Jan 15;1382(1):80-90.
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Author
- Koo JC, Lee SY, Chun HJ, Cheong YH, Choi JS, Kawabata S, Miyagi M, Tsunasawa S, Ha KS, Bae DW, Han CD, Lee BL, Cho MJ.