General Information

    • DRAMP ID

    • DRAMP00986

    • Peptide Name

    • Pn-AMP2 (PnAMP2; Plant defensin)

    • Source

    • Ipomoea nil (Japanese morning glory) (Pharbitis nil)

    • Family

    • Belongs to the hevein-like family

    • Gene

    • Not found

    • Sequence

    • QQCGRQASGRLCGNRLCCSQWGYCGSTASYCGAGCQSQCR

    • Sequence Length

    • 40

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal

    • Target Organism

      • Gram-positive bacterium: Bacillus subtilis (IC50=20 µg/ml).
      • Fungi (Medium A, Medium A+): Botrytis cinerea (IC50=2, 95 µg/ml), Colletotrichum langenarium (IC50=4, ND µg/ml), Sclerotinia sclerotiorum (IC50=3, >200 µg/ml), Fusarium oxysporum (IC50=2.5, >200 µg/ml), Rhizoctonia solani (IC50=75, >200 µg/ml), Phytophthora capsici (IC50=0.6, 150 µg/ml), Phytophthora parasitica (IC50=2, >200 µg/ml), Pythium spp. (IC50=2.5, ND µg/ml), Saccharomyces cerevisiae (IC50=8, >200 µg/ml).
      • NOTE! Medium A+ = Medium A supplemented with 5 mM CaCl2.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Chitin-binding

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00986 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00986.

Physicochemical Information

    • Formula

    • C165H262N60O56S8

    • Absent Amino Acids

    • DEFHIKMPV

    • Common Amino Acids

    • C

    • Mass

    • 4238.74

    • PI

    • 8.76

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +4

    • Boman Index

    • -84.99

    • Hydrophobicity

    • -0.513

    • Aliphatic Index

    • 27

    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 8980

    • Absorbance 280nm

    • 230.26

    • Polar Residues

    • 24

DRAMP00986

DRAMP00986 chydropathy plot

Comments Information

    • Miscellaneous

    • Its antimicrobial activity is strongly inhibited by divalent cations.

    • PTM

    • Contains four disulfide bonds 3-18; 12-24; 17-31; 35-39, and Pyrrolidone carboxylic acid at position 1.

Literature Information

  • ·Literature 1

    • Title

    • Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity.

    • Reference

    • Biochim Biophys Acta. 1998 Jan 15;1382(1):80-90.

    • Author

    • Koo JC, Lee SY, Chun HJ, Cheong YH, Choi JS, Kawabata S, Miyagi M, Tsunasawa S, Ha KS, Bae DW, Han CD, Lee BL, Cho MJ.