• DRAMP ID

    • DRAMP00986
    • Peptide Name

    • Pn-AMP2 (PnAMP2; Plant defensin)
    • Source

    • Ipomoea nil (Japanese morning glory) (Pharbitis nil)
    • Family

    • Belongs to the hevein-like family
    • Gene

    • N/A
    • Sequence

    • QQCGRQASGRLCGNRLCCSQWGYCGSTASYCGAGCQSQCR
    • Sequence Length

    • 40
    • Evidence code

    • Protein level
    • Biological Activity

    • Antifungal, Anti-Gram+, Antimicrobial
    • Target Organism

      • Gram-positive bacterium: Bacillus subtilis (IC50=20 µg/ml).
      • Fungi (Medium A, Medium A+): Botrytis cinerea (IC50=2, 95 µg/ml), Colletotrichum langenarium (IC50=4, ND µg/ml), Sclerotinia sclerotiorum (IC50=3, >200 µg/ml), Fusarium oxysporum (IC50=2.5, >200 µg/ml), Rhizoctonia solani (IC50=75, >200 µg/ml), Phytophthora capsici (IC50=0.6, 150 µg/ml), Phytophthora parasitica (IC50=2, >200 µg/ml), Pythium spp. (IC50=2.5, ND µg/ml), Saccharomyces cerevisiae (IC50=8, >200 µg/ml).
      • NOTE! Medium A+ = Medium A supplemented with 5 mM CaCl2.
    • Hemolytic Activity

      • N/A
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Chitin-binding
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Other Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • Bridge
    • Structure Description

    • N/A
    • DRAMP00986 helical wheel diagram
    • Formula

    • C165H262N60O56S8
    • Absent Amino Acids

    • DEFHIKMPV
    • Common Amino Acids

    • C
    • Mass

    • 4934.59
    • PI

    • 8.41
    • Basic Residues

    • 4
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 6
    • Boman Index

    • -84.99
    • Hydrophobicity

    • -51.25
    • Aliphatic Index

    • 27
    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 8980
    • Absorbance 280nm

    • 230.26
    • Polar Residues

    • 24

DRAMP00986

DRAMP00986 chydropathy plot
    • Miscellaneous

    • Its antimicrobial activity is strongly inhibited by divalent cations.
    • PTM

    • Contains four disulfide bonds 3-18; 12-24; 17-31; 35-39, and Pyrrolidone carboxylic acid at position 1.
  • Literature 1
    • Title

    • Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity.
    • Reference

    • Biochim Biophys Acta. 1998 Jan 15;1382(1):80-90.
    • Author

    • Koo JC, Lee SY, Chun HJ, Cheong YH, Choi JS, Kawabata S, Miyagi M, Tsunasawa S, Ha KS, Bae DW, Han CD, Lee BL, Cho MJ.