General Information

    • DRAMP ID

    • DRAMP00987

    • Peptide Name

    • Ee-CBPb (Hevein-like antimicrobial peptide; Plants)

    • Source

    • Euonymus europaeus (European spindle tree)

    • Family

    • Belongs to the hevein-like family

    • Gene

    • Not found

    • Sequence

    • QQCGRQAGNRRCANNLCCSQYGYCGRTNEYCCTSQGCQSQCRRCG

    • Sequence Length

    • 45

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Antifungal

    • Target Organism

      • Fungi: Alternaria brassicicola (IC50=3 µg/ml), Botrytis cinerea (IC50=1 µg/ml), Fusarium culmorum (IC50=3 µg/ml), Fusarium oxysporum f.sp.cubense (IC50=15 µg/ml), Fusarium oxysporum f.sp. Matthiolae (IC50=5 µg/ml), Mycosphaerella eumusae (IC50=6 µg/ml), Neurospora crassa (IC50=2 µg/ml), Phoma exigua (IC50=33 µg/ml), Phytophthora cryptogea (IC50=25 µg/ml), Pythium ultimum (IC50=33 µg/ml), Rhizoctonia solani (IC50=25 µg/ml).
      • Gram-positive bacteria: Bacillus megaterium (IC50=2 µg/ml), Sarcina lutea (IC50=7 µg/ml).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Chitin-binding

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00987 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00987.

Physicochemical Information

    • Formula

    • C190H306N74O67S10

    • Absent Amino Acids

    • DFHIKMPVW

    • Common Amino Acids

    • C

    • Mass

    • 5019.58

    • PI

    • 8.8

    • Basic Residues

    • 6

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 3

    • Net Charge

    • +5

    • Boman Index

    • -150.45

    • Hydrophobicity

    • -1.038

    • Aliphatic Index

    • 13.11

    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5095

    • Absorbance 280nm

    • 115.8

    • Polar Residues

    • 28

DRAMP00987

DRAMP00987 chydropathy plot

Comments Information

    • Function

    • Ee-CBPb possesses a very strong antifungal activity. Besides fungi, Ee-CBP1 also inhibits the growth of Gram-positive bacteria but not that of Gram-negative bacteria and yeasts.

    • PTM

    • Contains five disulfide bonds.

Literature Information

  • ·Literature 1

    • Title

    • Synergistic antifungal activity of two chitin-binding proteins from spindle tree (Euonymus europaeus L.).

    • Reference

    • Planta. 2004 Jun;219(2):221-232.

    • Author

    • Van den Bergh KP, Roug© P, Proost P, Coosemans J, Krouglova T, Engelborghs Y, Peumans WJ, Van Damme EJ.
  • ·Literature 2

    • Title

    • Five disulfide bridges stabilize a hevein-type antimicrobial peptide from the bark of spindle tree (Euonymus europaeus L.).

    • Reference

    • FEBS Lett. 2002 Oct 23;530(1-3):181-185.

    • Author

    • Van den Bergh KP, Proost P, Van Damme J, Coosemans J, Van Damme EJ, Peumans WJ.