• DRAMP ID

    • DRAMP00987
    • Peptide Name

    • Ee-CBPb (Hevein-like antimicrobial peptide; Plants)
    • Source

    • Euonymus europaeus (European spindle tree)
    • Family

    • Belongs to the hevein-like family
    • Gene

    • N/A
    • Sequence

    • QQCGRQAGNRRCANNLCCSQYGYCGRTNEYCCTSQGCQSQCRRCG
    • Sequence Length

    • 45
    • Swiss_Prot Entry

    • No entry found
    • Evidence code

    • N/A
    • Biological Activity

    • Antibacterial, Antifungal, Anti-Gram+, Antimicrobial
    • Target Organism

      • Fungi: Alternaria brassicicola (IC50=3 µg/ml), Botrytis cinerea (IC50=1 µg/ml), Fusarium culmorum (IC50=3 µg/ml), Fusarium oxysporum f.sp.cubense (IC50=15 µg/ml), Fusarium oxysporum f.sp. Matthiolae (IC50=5 µg/ml), Mycosphaerella eumusae (IC50=6 µg/ml), Neurospora crassa (IC50=2 µg/ml), Phoma exigua (IC50=33 µg/ml), Phytophthora cryptogea (IC50=25 µg/ml), Pythium ultimum (IC50=33 µg/ml), Rhizoctonia solani (IC50=25 µg/ml).
      • Gram-positive bacteria: Bacillus megaterium (IC50=2 µg/ml), Sarcina lutea (IC50=7 µg/ml).
    • Hemolytic Activity

      • N/A
    • Cytotoxicity

      • Not included yet
    • Binding Target

    • Chitin-binding
    • Linear/Cyclic

    • Not included yet
    • N-terminal Modification

    • Not included yet
    • C-terminal Modification

    • Not included yet
    • Other Modifications and Unusual Amino Acids

    • Not included yet
    • Stereochemistry

    • Not included yet
    • Structure

    • N/A
    • Structure Description

    • N/A
    • DRAMP00987 helical wheel diagram
    • Formula

    • C190H306N74O67S10
    • Absent Amino Acids

    • DFHIKMPVW
    • Common Amino Acids

    • C
    • Mass

    • 5804.6
    • PI

    • 8.42
    • Basic Residues

    • 6
    • Acidic Residues

    • 1
    • Hydrophobic Residues

    • 3
    • Boman Index

    • -150.45
    • Hydrophobicity

    • -103.78
    • Aliphatic Index

    • 13.11
    • Half Life

      • Mammalian:0.8 hour
      • Yeast:10 min
      • E.coli:>10 hour
    • Extinction Coefficient Cystines

    • 5095
    • Absorbance 280nm

    • 115.8
    • Polar Residues

    • 28

DRAMP00987

DRAMP00987 chydropathy plot
    • Function

    • Ee-CBPb possesses a very strong antifungal activity. Besides fungi, Ee-CBP1 also inhibits the growth of Gram-positive bacteria but not that of Gram-negative bacteria and yeasts.
    • PTM

    • Contains five disulfide bonds.
  • Literature 1
    • Title

    • Synergistic antifungal activity of two chitin-binding proteins from spindle tree (Euonymus europaeus L.).
    • Reference

    • Planta. 2004 Jun;219(2):221-232.
    • Author

    • Van den Bergh KP, Roug© P, Proost P, Coosemans J, Krouglova T, Engelborghs Y, Peumans WJ, Van Damme EJ.
  • Literature 2
    • Title

    • Five disulfide bridges stabilize a hevein-type antimicrobial peptide from the bark of spindle tree (Euonymus europaeus L.).
    • Reference

    • FEBS Lett. 2002 Oct 23;530(1-3):181-185.
    • Author

    • Van den Bergh KP, Proost P, Van Damme J, Coosemans J, Van Damme EJ, Peumans WJ.