General Information

    • DRAMP ID

    • DRAMP01444

    • Peptide Name

    • Nigrocin-1 (Frogs, amphibians, animals)

    • Source

    • Pelophylax nigromaculatus (Black-spotted frog) (Rana nigromaculata) (frog skin active peptide)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLLDSIKGMAISAGKGALQNLLKVASCKLDKTC

    • Sequence Length

    • 33

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.11682065]Gram-positive bacterium: Micrococcus luteus(MIC=2.5μg/ml);
      • Gram-negative bacteria: Shigella dysenteriae(MIC=10μg/ml), Klebsiella pneumoniae(MIC=10μg/ml), Pseudomonas aeruginosa(MIC=75μg/ml), Salmonella typhimurium(MIC=22.5μg/ml), Proteus mirabilis(MIC>200μg/ml), Serratia marcescens(MIC>200μg/ml);
      • Yeast: Candida albicans(MIC=100μg/ml).

    • Hemolytic Activity

      • [Ref:11682065]1.1% hemolytic activity at 100μg/ml against human red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01444 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01444.

Physicochemical Information

    • Formula

    • C144H256N40O44S3

    • Absent Amino Acids

    • EFHPRWY

    • Common Amino Acids

    • L

    • Mass

    • 3348.04

    • PI

    • 9.24

    • Basic Residues

    • 5

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 13

    • Net Charge

    • +3

    • Boman Index

    • -10.83

    • Hydrophobicity

    • 0.361

    • Aliphatic Index

    • 115.45

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.91

    • Polar Residues

    • 11

DRAMP01444

DRAMP01444 chydropathy plot

Comments Information

    • Function

    • Shows antibacterial activity against both Gram-positive and Gram-negative bacteria and against the fungus C. albicans. Has weak hemolytic activity.

    • Tissue specificity

    • Expressed by the skin dorsal glands.

    • PTM

    • Contains one disulfide bond 27-33. (By similarity)

Literature Information

  • ·Literature 1

    • Title

    • Structural study of novel antimicrobial peptides, nigrocins, isolated from Rana nigromaculata.

    • Reference

    • FEBS Lett. 2001 Oct 19;507(1):95-100.

    • Author

    • Park S, Park SH, Ahn HC, Kim S, Kim SS, Lee BJ, Lee BJ.