General Information

    • DRAMP ID

    • DRAMP01639

    • Peptide Name

    • Dermaseptin-1 (DShypo01, DPh-1; Frogs, amphibians, animals)

    • Source

    • Phyllomedusa hypochondrialis (Orange-legged leaf frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLWSTIKNVGKEAAIAAGKAALGAL

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antiprotozoal

    • Target Organism

      • [Ref.16963159]Gram-positive bacteria: Micrococcus luteus A270 (MIC=6 µM), Staphylococcus aureus ATCC 6538 (MIC=6 µM);
      • Gram-negative bacteria: Escherichia coli SBS 363 (MIC=6 µM), Pseudomonas aeruginosa ATCC 14502 (MIC=6.6 µM).

    • Hemolytic Activity

      • [Ref:16844081]Non-hemolytic activity upto 53 μM against human red blood cells;
      • [Ref.16963159]Non-hemolytic activity at 6 μM against erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01639 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C109H184N30O31

    • Absent Amino Acids

    • CDFHMPQRY

    • Common Amino Acids

    • A

    • Mass

    • 2410.84

    • PI

    • 9.7

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +2

    • Boman Index

    • 11.33

    • Hydrophobicity

    • 0.58

    • Aliphatic Index

    • 117.6

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 229.17

    • Polar Residues

    • 7

DRAMP01639

DRAMP01639 chydropathy plot

Comments Information

    • Function

    • Has antibacterial activity against Gram-negative bacteria and Gram-positive bacteria. Also has antiprotozoal activity against L. amazonensis. No hemolytic activity.

    • Tissue specificity

    • Expressed by the skin glands.

    • PTM

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Novel dermaseptins from Phyllomedusa hypochondrialis (Amphibia).

    • Reference

    • Biochem Biophys Res Commun. 2006 Sep 1;347(3):739-746.

    • Author

    • Brand GD, Leite JR, de Sá Mandel SM, Mesquita DA, Silva LP, Prates MV, Barbosa EA, Vinecky F, Martins GR, Galasso JH, Kuckelhaus SA, Sampaio RN, Furtado JR Jr, Andrade AC, Bloch C Jr.
  • ·Literature 2

    • Title

    • Isolation and biochemical characterization of peptides presenting antimicrobial activity from the skin of Phyllomedusa hypochondrialis.

    • Reference

    • Peptides. 2006 Dec;27(12):3092-3099.

    • Author

    • Conceição K, Konno K, Richardson M, Antoniazzi MM, Jared C, Daffre S, Camargo AC, Pimenta DC.