General Information

    • DRAMP ID

    • DRAMP01643

    • Peptide Name

    • Dermaseptin-5 (DShypo05, DS 01; Frogs, amphibians, animals)

    • Source

    • Phyllomedusa hypochondrialis (Orange-legged leaf frog)

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • GLWSTIKQKGKEAAIAAAKAAGQAALGAL

    • Sequence Length

    • 29

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antiprotozoal

    • Target Organism

      • [Ref.8306981]Gram-negative bacterium: Escherichia coli (MIC=6.4 µM);
      • Gram-positive bacterium: Staphylococcus aureus (MIC=3.2 µM).

    • Hemolytic Activity

      • [Ref:8306981]Non-hemolytic activity against rabbit red blood cells.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01643 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C125H212N36O36

    • Absent Amino Acids

    • CDFHMNPRVY

    • Common Amino Acids

    • A

    • Mass

    • 2795.28

    • PI

    • 10

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 16

    • Net Charge

    • +3

    • Boman Index

    • 2.73

    • Hydrophobicity

    • 0.286

    • Aliphatic Index

    • 101.72

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 196.43

    • Polar Residues

    • 6

DRAMP01643

DRAMP01643 chydropathy plot

Comments Information

    • Function

    • Has antibacterial activity against Gram-negative bacteria and Gram-positive bacteria. Also has antiprotozoal activity against L. amazonensis. No hemolytic activity.

    • Tissue specificity

    • Expressed by the skin glands.

    • PTM

    • C-terminal amidation.

Literature Information

  • ·Literature 1

    • Title

    • Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta. Anti-Trypanosoma cruzi activity without cytotoxicity to mammalian cells.

    • Reference

    • J Biol Chem. 2002 Dec 20;277(51):49332-49340.

    • Author

    • Brand GD, Leite JR, Silva LP, Albuquerque S, Prates MV, Azevedo RB, Carregaro V, Silva JS, Sá VC, Brandão RA, Bloch C Jr.
  • ·Literature 2

    • Title

    • Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta: Secondary structure, antimicrobial activity, and mammalian cell toxicity.

    • Reference

    • Comp Biochem Physiol A Mol Integr Physiol. 2008 Nov;151(3):336-343.

    • Author

    • Leite JRSA, Brand GD, Silva LP, Kückelhaus SAS, Bento WRC, Araújo ALT, Martins GR, Lazzari AM, Bloch C Jr.