General Information

    • DRAMP ID

    • DRAMP01785

    • Peptide Name

    • Temporin-CPa (Frogs, amphibians, animals)

    • Source

    • Lithobates capito (New World frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • IPPFIKKVLTTVF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.19635516]Gram-negative bacterium: Escherichia coli ATCC 25726 (MIC=25 µM);
      • Gram-positive bacterium: Staphylococcus aureus ATCC 25923 (MIC=50 µM);
      • Yeast: Candida albicans ATCC 90028 (MIC=25 µM).

    • Hemolytic Activity

      • [Ref:19635516]LC50=220 μM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Free

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01785 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C76H123N15O16

    • Absent Amino Acids

    • ACDEGHMNQRSWY

    • Common Amino Acids

    • FIKPTV

    • Mass

    • 1502.9

    • PI

    • 10

    • Basic Residues

    • 2

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +2

    • Boman Index

    • 12.56

    • Hydrophobicity

    • 1.108

    • Aliphatic Index

    • 134.62

    • Half Life

      • Mammalian:20 hour
      • Yeast:30 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP01785

Comments Information

    • Function

    • For this series of peptides, there were not enough material for antimicrobial assays. It displayed hemolytic activity (human red blood cells) at LC50=220 uM.

Literature Information

  • ·Literature 1

    • Title

    • Antimicrobial peptides from the skin secretions of the New World frogs Lithobates capito and Lithobates warszewitschii (Ranidae).

    • Reference

    • Peptides. 2009 Oct;30(10):1775-1781.

    • Author

    • Conlon JM, Meetani MA, Coquet L, Jouenne T, Leprince J, Vaudry H, Kolodziejek J, Nowotny N, King JD.