General Information

    • DRAMP ID

    • DRAMP01833

    • Peptide Name

    • Buforin-EC (Frogs, amphibians, animals)

    • Source

    • Euphlyctis cyanophlyctis (Skittering frog)

    • Family

    • Belongs to the histone H2A family

    • Gene

    • Not found

    • Sequence

    • RAGLKFPVGRVHRLLR

    • Sequence Length

    • 16

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Swiss_Prot Entry C0HJB7]Gram-negative bacteria: Escherichia coli HP101BA (MIC=9.6 µM), Klebsiella pneumoniae PTCC1388 (MIC=10.6 µM);
      • Gram-positive bacteria: Micrococcus luteus PTCC1625 (MIC=6.0 µM), Staphylococcus aureus PTCC1431 (MIC=8.1 µM).

    • Hemolytic Activity

      • [Ref.Int J Pept Res Ther(2012)18;107.]<3% hemolytic activity at 60 µg/ml against human red blood cells

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01833 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C85H147N31O17

    • Absent Amino Acids

    • CDEIMNQSTWY

    • Common Amino Acids

    • R

    • Mass

    • 1875.3

    • PI

    • 12.48

    • Basic Residues

    • 6

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +6

    • Boman Index

    • -40.38

    • Hydrophobicity

    • -0.194

    • Aliphatic Index

    • 115.63

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP01833

Comments Information

    • Function

    • Has antibacterial activity. Has no or very limited (<3%) hemolytic activity at concentrations of 15 µg/ml and 60 µg/ml, respectively.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Identification and characterization of novel antibacterial peptides from skin secretions of Euphlyctis cyanophlyctis.

    • Reference

    • Int. J. Pept. Res. Ther. 2012;18:107-115.

    • Author

    • Asoodeh A, Ghorani-Azam A, Chamani JK.