General Information

    • DRAMP ID

    • DRAMP01844

    • Peptide Name

    • Ascaphin-5 (Frogs, amphibians, animals)

    • Source

    • Ascaphus truei (Coastal tailed frog)

    • Family

    • Belongs to the ascaphin family

    • Gene

    • Not found

    • Sequence

    • GIKDWIKGAAKKLIKTVASNIANQ

    • Sequence Length

    • 24

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.15207717]Gram-negative bacteria: Escherichia coli (MIC=6 µM), Pseudomonas aeruginosa (MIC=13 µM), Enterobacter cloacae (MIC=6 µM), Klebsiella pneumoniae (MIC=13 µM), Proteus mirabilis (MIC>100 µM);
      • Gram-positive bacteria: Staphylococcus aureus (MIC=50 µM), Staphylococcus epidermidis (MIC=13 µM), Enterococcus faecalis (MIC>100 µM), Streptococcus Group B (MIC=6 µM);
      • Yeast: Candida albicans (MIC=100 µM).

    • Hemolytic Activity

      • [Ref.15207717]Not found

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01844 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C116H199N33O32

    • Absent Amino Acids

    • CEFHMPRY

    • Common Amino Acids

    • K

    • Mass

    • 2568.06

    • PI

    • 10.18

    • Basic Residues

    • 5

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +4

    • Boman Index

    • -21.17

    • Hydrophobicity

    • -0.146

    • Aliphatic Index

    • 110

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 239.13

    • Polar Residues

    • 6

DRAMP01844

DRAMP01844 chydropathy plot

Comments Information

    • Function

    • Antimicrobial peptide that shows higher potency against Gram-negative bacteria than against Gram-positive bacteria. Tissue specificity

Literature Information

  • ·Literature 1

    • Title

    • The ascaphins: a family of antimicrobial peptides from the skin secretions of the most primitive extant frog, Ascaphus truei.

    • Reference

    • Biochem Biophys Res Commun. 2004 Jul 16;320(1):170-175.

    • Author

    • Conlon JM, Sonnevend A, Davidson C, Smith DD, Nielsen PF.