General Information

    • DRAMP ID

    • DRAMP01847

    • Peptide Name

    • Ascaphin-8 (Frogs, amphibians, animals)

    • Source

    • Ascaphus truei (Coastal tailed frog)

    • Family

    • Belongs to the ascaphin family

    • Gene

    • Not found

    • Sequence

    • GFKDLLKGAAKALVKTVLF

    • Sequence Length

    • 19

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antiviral

    • Target Organism

      • [Ref.15207717]Gram-negative bacterium: Escherichia coli (MIC=6 µM);
      • Gram-positive bacterium: Staphylococcus aureus (MIC=6 µM).

    • Hemolytic Activity

      • [Ref.15207717]HC50=50 μM against human erythrocytes

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01847 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C97H163N23O23

    • Absent Amino Acids

    • CEHIMNPQRSWY

    • Common Amino Acids

    • KL

    • Mass

    • 2019.5

    • PI

    • 10

    • Basic Residues

    • 4

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +3

    • Boman Index

    • 7.54

    • Hydrophobicity

    • 0.737

    • Aliphatic Index

    • 128.42

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP01847

DRAMP01847 chydropathy plot

Comments Information

    • Function

    • Antimicrobial peptide that shows similar potency against Gram-negative bacteria and Gram-positive bacteria. Has hemolytic activity. Tissue specificity

Literature Information

  • ·Literature 1

    • Title

    • The ascaphins: a family of antimicrobial peptides from the skin secretions of the most primitive extant frog, Ascaphus truei.

    • Reference

    • Biochem Biophys Res Commun. 2004 Jul 16;320(1):170-175.

    • Author

    • Conlon JM, Sonnevend A, Davidson C, Smith DD, Nielsen PF.