General Information

    • DRAMP ID

    • DRAMP01918

    • Peptide Name

    • Brevinin-1PLb (Frogs, amphibians, animals)

    • Source

    • Rana palustris (Pickerel frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLPLIAGLAANFLPKIFCAITKKC

    • Sequence Length

    • 24

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-positive bacterium: Staphylococcus aureus (MIC=9 µM);
      • Gram-negative bacterium: Escherichia coli(MIC=26 µM).
      • Yeast: Candida albicans (MIC=6 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not included yet

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Not included yet

    • N-terminal Modification

    • Not included yet

    • C-terminal Modification

    • Not included yet

    • Nonterminal Modifications and Unusual Amino Acids

    • Not included yet

    • Stereochemistry

    • Not included yet

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01918 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C125H202N28O27S2

    • Absent Amino Acids

    • DEHMQRSVWY

    • Common Amino Acids

    • AL

    • Mass

    • 2593.27

    • PI

    • 9.39

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +3

    • Boman Index

    • 28.26

    • Hydrophobicity

    • 1.242

    • Aliphatic Index

    • 130.42

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 5

DRAMP01918

DRAMP01918 chydropathy plot

Comments Information

    • Function

    • Has antimicrobial activity.

    • Tissue specificity

    • Expressed by the skin glands.

Literature Information

  • ·Literature 1

    • Title

    • Rapid identification of precursor cDNAs encoding five structural classes of antimicrobial peptides from pickerel frog (Rana palustris) skin secretion by single step shotgun cloning.

    • Reference

    • Peptides. 2007 Aug;28(8):1605-1610.

    • Author

    • Zhou M, Wang L, Owens DE, Chen T, Walker B, Shaw C.
  • ·Literature 2

    • Title

    • Multiple antimicrobial peptides and peptides related to bradykinin and neuromedin N isolated from skin secretions of the pickerel frog, Rana palustris.

    • Reference

    • Biochim Biophys Acta. 2000 Nov 30;1543(1):95-105.

    • Author

    • Basir YJ, Knoop FC, Dulka J, Conlon JM.