General Information

    • DRAMP ID

    • DRAMP01940

    • Peptide Name

    • Brevinin-1CHa (Frogs, amphibians, animals)

    • Source

    • Rana chiricahuensis

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLPIIAGVAAKVLPKLFCAITKKC

    • Sequence Length

    • 24

    • Protein Existence

    • Homology

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • Gram-negative bacteria: E. coli (MIC=10 µM), K. pneumoniae (MIC=40 µM), P. aeruginosa (MIC=20 µM);
      • Gram-positive bacterium: S. aureus (MIC=2.5 µM).
      • Yeast: C. albicans (MIC=2.5 µM).(Ref.2)

    • Hemolytic Activity

      • [Ref.21262304] LC50=5 µM against human erythrocytes

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP01940 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP01940.

Physicochemical Information

    • Formula

    • C122H206N28O26S2

    • Absent Amino Acids

    • DEHMNQRSWY

    • Common Amino Acids

    • AK

    • Mass

    • 2545.27

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +4

    • Boman Index

    • 29.53

    • Hydrophobicity

    • 1.3

    • Aliphatic Index

    • 138.33

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 4

DRAMP01940

DRAMP01940 chydropathy plot

Comments Information

    • PTM

    • Contains disulfide bond.

Literature Information

  • ·Literature 1

    • Title

    • Selection for antimicrobial peptide diversity in frogs leads to gene duplication and low allelic variation.

    • Reference

    • J Mol Evol. 2007 Nov;65(5):605-615.

    • Author

    • Tennessen JA, Blouin MS.
  • ·Literature 2

    • Title

    • Characterization of antimicrobial peptides in skin secretions from discrete populations of Lithobates chiricahuensis (Ranidae) from central and southern Arizona.

    • Reference

    • Peptides. 2011 Apr;32(4):664-669.

    • Author

    • Conlon JM, Mechkarska M, Coquet L, Jouenne T, Leprince J, Vaudry H, Kolodziejek J, Nowotny N, King JD.