General Information
-
DRAMP ID
- DRAMP01956
-
Peptide Name
- Brevinin-2PTb (Frogs, amphibians, animals)
-
Source
- Rana picturata (Malaysian fire frog) (Hylarana picturata)
-
Family
- Belongs to the frog skin active peptide family (Brevinin subfamily)
-
Gene
- Not found
-
Sequence
- GFKGAFKNVMFGIAKSAGKSALNALACKIDKSC
-
Sequence Length
- 33
-
UniProt Entry
- P0C8T4
-
Protein Existence
- Protein level
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- Gram-positive bacterium: Staphylococcus aureus ATCC 25923 (MIC=9 µM);
- Gram-negative bacterium: Escherichia coli ATCC 25726 (MIC=9 µM).
-
Hemolytic Activity
-
- No hemolysis information or data found in the reference(s) presented in this entry
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Cyclic
-
N-terminal Modification
- Free
-
C-terminal Modification
- Cyclization (Cys27 and Cys33)
-
Nonterminal Modifications and Unusual Amino Acids
- Disulfide bond between Cys27 and Cys33.
-
Stereochemistry
- L
-
Structure
- Not found
-
Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- Please click DRAMP01956_predicted_structure.pdb to download.
Physicochemical Information
-
Formula
- C150H247N41O41S3
Absent Amino Acids
- EHPQRTWY
Common Amino Acids
- AK
Mass
- 3377.04
PI
- 9.79
Basic Residues
- 6
Acidic Residues
- 1
Hydrophobic Residues
- 14
Net Charge
- +5
-
Boman Index
- -13.31
Hydrophobicity
- 0.273
Aliphatic Index
- 74.24
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 125
Absorbance 280nm
- 3.91
Polar Residues
- 11
DRAMP01956
Comments Information
Function
- Has antibacterial activity.
Tissue specificity
- Expressed by the skin glands.
PTM
- Contains one disulfide bond 27-33.
Literature Information
- ·Literature 1
-
Title
- Characterization of antimicrobial peptides from the skin secretions of the Malaysian frogs, Odorrana hosii and Hylarana picturata (Anura:Ranidae).
-
Pubmed ID
- 18621071
-
Reference
- Toxicon. 2008 Sep 1;52(3):465-473.
-
Author
- Conlon JM, Kolodziejek J, Nowotny N, Leprince J, Vaudry H, Coquet L, Jouenne T, King JD.