General Information

    • DRAMP ID

    • DRAMP02009

    • Peptide Name

    • Brevinin-1 (Frogs, amphibians, animals)

    • Source

    • Rana brevipoda porsa (Japanese frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • FLPVLAGIAAKVVPALFCKITKKC

    • Sequence Length

    • 24

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.1449472]Gram-positive bacterium: Staphylococcus aureus (MIC=8 µg/ml);
      • Gram-negative bacterium: Escherichia coli (MIC=34 µg/ml).

    • Hemolytic Activity

      • [Ref.1449472] high hemolytic activity

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys18 and Cys24)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys18 and Cys24.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02009 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C121H204N28O26S2

    • Absent Amino Acids

    • DEHMNQRSWY

    • Common Amino Acids

    • AK

    • Mass

    • 2531.24

    • PI

    • 9.7

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 14

    • Net Charge

    • +4

    • Boman Index

    • 28.65

    • Hydrophobicity

    • 1.288

    • Aliphatic Index

    • 134.17

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 5.43

    • Polar Residues

    • 4

DRAMP02009

DRAMP02009 chydropathy plot

Comments Information

    • Function

    • Shows antibacterial activity against representative Gram-negative and Gram-positive bacterial species, and a very high hemolytic activity.

Literature Information

  • ·Literature 1

    • Title

    • Brevinin-1 and -2, unique antimicrobial peptides from the skin of the frog, Rana brevipoda porsa.

    • Reference

    • Biochem Biophys Res Commun. 1992 Nov 30;189(1):184-190.

    • Author

    • Morikawa N, Hagiwara K, Nakajima T.