General Information

    • DRAMP ID

    • DRAMP02025

    • Peptide Name

    • Brevinin-2DYe (Brevinin-2CDYa; Frogs, amphibians, animals)

    • Source

    • Rana dybowskii (Dybovsky's frog) (Korean brown frog)

    • Family

    • Belongs to the frog skin active peptide family (Brevinin subfamily)

    • Gene

    • Not found

    • Sequence

    • GLFSVVTGVLKAVGKNVAKNVGGSLLEQLKCKISGGC

    • Sequence Length

    • 37

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • Gram-positive bacterium: Staphylococcus aureus (MIC=15 µM);
      • Gram-negative bacterium: Escherichia coli (MIC=30 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization (Cys31 and Cys37)

    • Nonterminal Modifications and Unusual Amino Acids

    • Disulfide bond between Cys31 and Cys37.

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP02025 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

Physicochemical Information

    • Formula

    • C162H281N45O47S2

    • Absent Amino Acids

    • DHMPRWY

    • Common Amino Acids

    • G

    • Mass

    • 3675.41

    • PI

    • 9.6

    • Basic Residues

    • 5

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 15

    • Net Charge

    • +4

    • Boman Index

    • 3.35

    • Hydrophobicity

    • 0.56

    • Aliphatic Index

    • 115.68

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 125

    • Absorbance 280nm

    • 3.47

    • Polar Residues

    • 15

DRAMP02025

DRAMP02025 chydropathy plot

Comments Information

    • Function

    • Active against the Gram-positive bacteria

Literature Information

  • ·Literature 1

    • Title

    • Cytolytic peptides belonging to the brevinin-1 and brevinin-2 families isolated from the skin of the Japanese brown frog, Rana dybowskii.

    • Reference

    • Toxicon. 2007 Nov;50(6):746-756.

    • Author

    • Conlon JM, Kolodziejek J, Nowotny N, Leprince J, Vaudry H, Coquet L, Jouenne T, Iwamuro S.
  • ·Literature 2

    • Title

    • Purification and characterization of antimicrobial peptides from the skin secretion of Rana dybowskii.

    • Reference

    • Peptides. 2007 Aug;28(8):1532-1539.

    • Author

    • Kim SS, Shim MS, Chung J, Lim DY, Lee BJ.